Vitamin B6 in plasma and cerebrospinal fluid of children

Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce. B6 vitamer concentrations in simulta...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0120972-e0120972
Main Authors: Albersen, Monique, Bosma, Marjolein, Jans, Judith J M, Hofstede, Floris C, van Hasselt, Peter M, de Sain-van der Velden, Monique G M, Visser, Gepke, Verhoeven-Duif, Nanda M
Format: Article
Language:English
Subjects:
Acids
Adolescent
Anticonvulsants - therapeutic use
Blood plasma
Brain
Cerebrospinal fluid
Child
Child, Preschool
Children
Chromatography
Chromatography, Liquid
Cross-Sectional Studies
Dehydrogenases
Disorders
Drug dosages
Drugs
Epilepsy
Ethics
Female
Fluid
Fluids
Genetics
Homocysteine
Humans
Infant
Intellectual disabilities
Intellectual Disability - drug therapy
Intellectual Disability - metabolism
Liquid chromatography
Male
Mass spectrometry
Mass spectroscopy
Metabolic disorders
Metabolism
Metabolites
Nutrient deficiency
Phosphatase
Phosphates
Physiological aspects
Pyridoxal - blood
Pyridoxal - cerebrospinal fluid
Pyridoxal Phosphate - blood
Pyridoxal Phosphate - cerebrospinal fluid
Pyridoxamine - cerebrospinal fluid
Pyridoxic Acid - blood
Pyridoxic Acid - cerebrospinal fluid
Pyridoxine
Scientific imaging
Substance abuse treatment
Tandem Mass Spectrometry
Vitamin B 6 - blood
Vitamin B 6 - cerebrospinal fluid
Vitamin B6
Vitamin deficiency
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Summary:Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce. B6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF) of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem mass spectrometry. For ethical reasons, CSF samples could not be obtained from healthy children. The influence of sex, age, epilepsy and treatment with anti-epileptic drugs, were investigated. The B6 vitamer composition of plasma (pyridoxal phosphate (PLP) > pyridoxic acid > pyridoxal (PL)) differed from that of CSF (PL > PLP > pyridoxic acid > pyridoxamine). Strong correlations were found for B6 vitamers in and between plasma and CSF. Treatment with anti-epileptic drugs resulted in decreased concentrations of PL and PLP in CSF. We provide concentrations of all B6 vitamers in plasma and CSF of children with intellectual disability (±epilepsy), which can be used in the investigation of known and novel disorders associated with vitamin B6 metabolism as well as in monitoring of the biochemical effects of treatment with vitamin B6.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0120972