Osteogenic differentiation of human mesenchymal stem cells in 3-D Zr-Si organic-inorganic scaffolds produced by two-photon polymerization technique

Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds for bone tissue engineering. Zr-Si scaffolds with 150, 200, and 250 μm pore sizes are seeded with human bone marrow stem cells (hBMSCs) and human adipose tissue derived stem cells (hASCs) and cultured in osteoinduc...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-02, Vol.10 (2), p.e0118164-e0118164
Main Authors: Koroleva, Anastasia, Deiwick, Andrea, Nguyen, Alexander, Schlie-Wolter, Sabrina, Narayan, Roger, Timashev, Peter, Popov, Vladimir, Bagratashvili, Viktor, Chichkov, Boris
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Aging
Alcohols
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Autografts
Biocompatibility
Biomedical engineering
Biomedical materials
Bone growth
Bone marrow
Bone matrix
Calcium
Calcium - metabolism
Cell Culture Techniques - methods
Cell Differentiation
Cell growth
Cell Proliferation
Cells, Cultured
Chemistry Techniques, Synthetic - methods
Differentiation (biology)
Elastic Modulus
Electron microscopy
Engineering
Fabrication
Gene expression
Hardness
Humans
Inorganic Chemicals - chemistry
Lasers
Mechanical properties
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchymal Stem Cells - ultrastructure
Mesenchyme
Microscopy, Electron, Scanning
Mineralization
Modulus of elasticity
Nanoindentation
Nanotechnology
Organic Chemicals - chemistry
Osteocalcin
Osteocalcin - metabolism
Osteogenesis
Polymerization
Pore size
Porosity
Reproducibility of Results
Scaffolds
Scanning electron microscopy
Scanning transmission electron microscopy
Silicon - chemistry
Skin & tissue grafts
Staining
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Stem Cells - ultrastructure
Time Factors
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Transplants & implants
Zirconium
Zirconium - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds for bone tissue engineering. Zr-Si scaffolds with 150, 200, and 250 μm pore sizes are seeded with human bone marrow stem cells (hBMSCs) and human adipose tissue derived stem cells (hASCs) and cultured in osteoinductive and control media for three weeks. Osteogenic differentiation of hASCs and hBMSCs and formation of bone matrix is comparatively analyzed via alkaline phosphatase activity (ALP), calcium quantification, osteocalcin staining and scanning electron microscopy (SEM). It is observed that the 150 μm pore size Zr-Si scaffolds support the strongest matrix mineralization, as confirmed by calcium deposition. Analysis of ALP activity, osteocalcin staining and SEM observations of matrix mineralization reveal that mesenchymal stem cells cultured on 3-D scaffolds without osteogenic stimulation spontaneously differentiate towards osteogenic lineage. Nanoindentation measurements show that aging of the 2PP-produced Zr-Si scaffolds in aqueous or alcohol media results in an increase in the scaffold Young's modulus and hardness. Moreover, accelerated formation of bone matrix by hASCs is noted, when cultured on the scaffolds with lower Young's moduli and hardness values (non aged scaffolds) compared to the cells cultured on scaffolds with higher Young's modulus and hardness values (aged scaffolds). Presented results support the potential application of Zr-Si scaffolds for autologous bone tissue engineering.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0118164