Nanomiemgel--a novel drug delivery system for topical application--in vitro and in vivo evaluation

The objective of this study was to formulate and evaluate a unique matrix mixture (nanomiemgel) of nanomicelle and nanoemulsion containing aceclofenac and capsaicin using in vitro and in vivo analyses and to compare it to a marketed formulation (Aceproxyvon). Nanomicelles were prepared using Vitamin...

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Bibliographic Details
Published in:PloS one 2014-12, Vol.9 (12), p.e115952-e115952
Main Authors: Somagoni, Jaganmohan, Boakye, Cedar H A, Godugu, Chandraiah, Patel, Apurva R, Mendonca Faria, Henrique Antonio, Zucolotto, Valtencir, Singh, Mandip
Format: Article
Language:English
Subjects:
Absorption
Administration, Cutaneous
Aminoquinolines - pharmacology
Aminoquinolines - therapeutic use
Animals
Arthritis
Atomic force microscopy
Biomedical materials
Capsaicin
Capsaicin - pharmacology
Capsaicin - therapeutic use
Cell Proliferation - drug effects
Controlled release
Cytokines
Diclofenac - analogs & derivatives
Diclofenac - pharmacology
Diclofenac - therapeutic use
Disease Models, Animal
Drug delivery
Drug Delivery Systems
Drug Stability
Drugs
Ear
Emulsions
Evaporation
Gels - chemistry
High-performance liquid chromatography
House mouse
Humans
Imiquimod
Immunohistochemistry
In vitro methods and tests
In Vitro Techniques
In vivo methods and tests
Inflammation
Inflammation - pathology
Keratinocytes - drug effects
Keratinocytes - pathology
Liquid chromatography
Lung cancer
Medicine and Health Sciences
Mice
Micelles
Microdialysis
Microscopy
Microscopy, Atomic Force
Nanoemulsions
Nanoparticles - chemistry
Narcotics
New combinations
Organ Size - drug effects
Penetration
Permeability
Pharmaceutical sciences
Pharmacy
Polyethylene glycol
Polyvinyl alcohol
Psoriasis
Psoriasis - drug therapy
Psoriasis - pathology
Rats
Rheology
Skin
Skin Absorption - drug effects
Spleen
Spleen - drug effects
Spleen - pathology
Staphylococcus infections
Surfactants
Tocopherol
Topical application
Transdermal drug delivery systems
Vitamin E
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Summary:The objective of this study was to formulate and evaluate a unique matrix mixture (nanomiemgel) of nanomicelle and nanoemulsion containing aceclofenac and capsaicin using in vitro and in vivo analyses and to compare it to a marketed formulation (Aceproxyvon). Nanomicelles were prepared using Vitamin E TPGS by solvent evaporation method and nanoemulsion was prepared by high-pressure homogenization method. In vitro drug release and human skin permeation studies were performed and analyzed using HPLC. The efficiency of nanomiemgel as a delivery system was investigated using an imiquimod-induced psoriatic like plaque model developed in C57BL/6 mice. Atomic Force Microscopy images of the samples exhibited a globular morphology with an average diameter of 200, 250 and 220 nm for NMI, NEM and NMG, respectively. Nanomiemgel demonstrated a controlled release drug pattern and induced 2.02 and 1.97-fold more permeation of aceclofenac and capsaicin, respectively than Aceproxyvon through dermatomed human skin. Nanomiemgel also showed 2.94 and 2.09-fold greater Cmax of aceclofenac and capsaicin, respectively than Aceproxyvon in skin microdialysis study in rats. The PASI score, ear thickness and spleen weight of the imiquimod-induced psoriatic-like plaque model were significantly (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0115952