Comparative transcriptome analysis reveals early pregnancy-specific genes expressed in peripheral blood of pregnant sows

Early and accurate diagnosis of pregnancy is important for effective management of an economical pig farm. Besides the currently available methods used in early diagnosis of sows, circulating nucleic acids in peripheral blood may contain some early pregnancy-specific molecular markers. For the first...

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Bibliographic Details
Published in:PloS one 2014-12, Vol.9 (12), p.e114036-e114036
Main Authors: Shen, Junye, Zhou, Chuanli, Zhu, Shien, Shi, Wenqing, Hu, Maishun, Fu, Xiangwei, Wang, Chuduan, Wang, Yachun, Zhang, Qin, Yu, Ying
Format: Article
Language:English
Subjects:
Acids
Analysis
Animal sciences
Animals
Annotations
Arachidonic acid
Biology and Life Sciences
Blood
Blood circulation
Bovidae
Breeding of animals
Cattle
Cell surface
Cellular signal transduction
Diagnosis
DNA microarrays
Endometrium - growth & development
Endometrium - metabolism
Engineering
Farm management
Female
G protein-coupled receptors
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes
Genetics
Genomics
Hogs
Humans
Kinases
Laboratories
Luteinizing hormone
Markers
Medicine and Health Sciences
Metabolism
Molecular chains
Nucleic acids
Oligonucleotide Array Sequence Analysis
Peripheral blood
Pork industry
Pregnancy
Pregnant women
Research and Analysis Methods
RNA, Messenger - biosynthesis
Signal Transduction
Signaling
Swine
Transcriptome - genetics
USP18 protein
Zoology
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Summary:Early and accurate diagnosis of pregnancy is important for effective management of an economical pig farm. Besides the currently available methods used in early diagnosis of sows, circulating nucleic acids in peripheral blood may contain some early pregnancy-specific molecular markers. For the first time, microarray analysis of peripheral blood from pregnant sows versus non-pregnant sows identified 127 up-regulated and 56 down-regulated genes at day 14 post-insemination. Gene Ontology annotation grouped the total differently expressed genes into 3 significantly enriched terms, cell surface receptor linked signal transduction, G-protein coupled receptor protein signaling pathway and regulation of vesicle-mediated transport. Signaling pathway analysis revealed the only one significantly changed pathway was arachidonic acid metabolism. Of the differently expressed genes, nine (including LPAR3, RXFP4, GALP, CBR1, CBR2, GPX6, USP18, LHB and NR5A1) were found to exert function related to early pregnancy processes. This study provides a clue that differentially abundant RNAs in maternal peripheral blood can help to identify the molecular markers of early pregnancy in pigs.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0114036