Changes in expression of aquaporin-4 and aquaporin-9 in optic nerve after crushing in rats

The purpose of this study was to determine the temporal and spatial changes in the expression of AQP4 and AQP9 in the optic nerve after it is crushed. The left optic nerves of rats were either crushed (crushed group) or sham operated (sham group), and they were excised before, and at 1, 2, 4, 7, and...

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Published in:PloS one 2014-12, Vol.9 (12), p.e114694-e114694
Main Authors: Suzuki, Hiroyuki, Oku, Hidehiro, Horie, Taeko, Morishita, Seita, Tonari, Masahiro, Oku, Kazuma, Okubo, Akiko, Kida, Teruyo, Mimura, Masashi, Fukumoto, Masanori, Kojima, Shota, Takai, Shinji, Ikeda, Tsunehiko
Format: Article
Language:English
Subjects:
Animals
Aquaporin 4
Aquaporin 4 - metabolism
Aquaporins
Aquaporins - metabolism
Astrocytes
Biology and Life Sciences
Brain research
Cell adhesion & migration
Crushing
Diabetes
Diabetic retinopathy
Edema
Edema - etiology
Glaucoma
Glial fibrillary acidic protein
Homeostasis
Immunohistochemistry
Intermediate filament proteins
Male
Moisture content
Nerve Crush - adverse effects
Nerve Crush - methods
Nerve Tissue Proteins - metabolism
Nerves
Optic nerve
Optic Nerve - metabolism
Optic Nerve - surgery
Optic Nerve Diseases - etiology
Optic Nerve Injuries - metabolism
Rats
Rats, Wistar
Research and Analysis Methods
Retina
Rodents
Water content
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Summary:The purpose of this study was to determine the temporal and spatial changes in the expression of AQP4 and AQP9 in the optic nerve after it is crushed. The left optic nerves of rats were either crushed (crushed group) or sham operated (sham group), and they were excised before, and at 1, 2, 4, 7, and 14 days later. Four optic nerves were pooled for each time point in both groups. The expression of AQP4 and AQP9 was determined by western blot analyses. Immunohistochemistry was used to determine the spatial expression of AQP4, AQP9, and GFAP in the optic nerve. Optic nerve edema was determined by measuring the water content in the optic nerve. The barrier function of the optic nerve vessels was determined by the extravasated Evans blue dye on days 7 and 14. The results showed that the expression of AQP4 was increased on day 1 but the level was significantly lower than that in the sham group on days 4 and 7 (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0114694