Activated human mast cells induce LOX-1-specific scavenger receptor expression in human monocyte-derived macrophages

Activated mast cells in atherosclerotic lesions degranulate and release bioactive compounds capable of regulating atherogenesis. Here we examined the ability of activated human primary mast cells to regulate the expression of the major scavenger receptors in cultured human primary monocyte-derived m...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-09, Vol.9 (9), p.e108352-e108352
Main Authors: Alanne-Kinnunen, Mervi, Lappalainen, Jani, Öörni, Katariina, Kovanen, Petri T
Format: Article
Language:English
Subjects:
Anticoagulants
Arteriosclerosis
Atherogenesis
Atherosclerosis
Bioactive compounds
Biology and Life Sciences
Blood & organ donations
CD36 antigen
CD36 Antigens - genetics
Cell culture
Cells, Cultured
Gene expression
Growth factors
Histamine
Histamine - metabolism
Histamine - pharmacology
Human performance
Humans
Immunoglobulins
Lesions
Lipids
Liquid oxygen
Low density lipoprotein
LOX-1 protein
Macrophages
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Mast cells
Mast Cells - cytology
Mast Cells - immunology
Medicine and Health Sciences
Monocytes
Receptors
Scavenger receptors
Scavenger Receptors, Class A - genetics
Scavenger Receptors, Class E - genetics
Scavenger Receptors, Class E - metabolism
Synergistic effect
Transforming Growth Factor beta1 - metabolism
Transforming Growth Factor beta1 - pharmacology
Transforming growth factor-a
Transforming growth factor-b
Transforming growth factor-b1
Tumor Necrosis Factor-alpha - metabolism
Tumor Necrosis Factor-alpha - pharmacology
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Activated mast cells in atherosclerotic lesions degranulate and release bioactive compounds capable of regulating atherogenesis. Here we examined the ability of activated human primary mast cells to regulate the expression of the major scavenger receptors in cultured human primary monocyte-derived macrophages (HMDMs). Components released by immunologically activated human primary mast cells induced a transient expression of lectin-like oxidized LDL receptor (LOX-1) mRNA in HMDMs, while the expression of two other scavenger receptors, MSR1 and CD36, remained unaffected. The LOX-1-inducing secretory components were identified as histamine, tumor necrosis factor alpha (TNF-α), and transforming growth factor beta (TGF-β1), which exhibited a synergistic effect on LOX-1 mRNA expression. Histamine induced a transient expression of LOX-1 protein. Mast cell -induced increase in LOX-1 expression was not associated with increased uptake of oxidized LDL by the macrophages. Mast cell-derived histamine, TNF-α, and TGF-β1 act in concert to induce a transient increase in LOX-1 expression in human primary monocyte-derived macrophages. The LOX-1-inducing activity potentially endows mast cells a hitherto unrecognized role in the regulation of innate immune reactions in atherogenesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0108352