Virulence and draft genome sequence overview of multiple strains of the swine pathogen Haemophilus parasuis

Haemophilus parasuis is the cause of Glässer's disease in swine, which is characterized by systemic infection resulting in polyserositis, meningitis, and arthritis. Investigation of this animal disease is complicated by the enormous differences in the severity of disease caused by H. parasuis s...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e103787-e103787
Main Authors: Brockmeier, Susan L, Register, Karen B, Kuehn, Joanna S, Nicholson, Tracy L, Loving, Crystal L, Bayles, Darrell O, Shore, Sarah M, Phillips, Gregory J
Format: Article
Language:English
Subjects:
Acids
Adhesins, Bacterial - genetics
Adhesins, Bacterial - immunology
Analysis
Animal diseases
Animals
Animals, Newborn
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Arthritis
Bioinformatics
Biology and Life Sciences
Cesarean Section
Chromosome Mapping
Colostrum
Deoxyribonucleic acid
Disseminated infection
DNA
Fimbriae Proteins - genetics
Fimbriae Proteins - immunology
Genome, Bacterial
Genomes
Genomic analysis
Genomics
Genotypes
Haemophilus
Haemophilus Infections - microbiology
Haemophilus Infections - mortality
Haemophilus Infections - pathology
Haemophilus Infections - veterinary
Haemophilus influenzae
Haemophilus parasuis - genetics
Haemophilus parasuis - immunology
Haemophilus parasuis - pathogenicity
Health aspects
Hemophilus infections
High-Throughput Nucleotide Sequencing
Hogs
Infection
Infections
Livestock
Medicine and Health Sciences
Meningitis
Metabolism
Morbidity
Nucleotide sequence
Pasteurellaceae
Pilin
Polyserositis
Preventive medicine
Proteins
Serotyping
Severity of Illness Index
Strains (organisms)
Suidae
Survival Analysis
Swine
Swine Diseases - microbiology
Swine Diseases - mortality
Swine Diseases - pathology
Vaccines
Veterinary colleges
Veterinary medicine
Virulence
Virulence (Microbiology)
Virulence factors
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Summary:Haemophilus parasuis is the cause of Glässer's disease in swine, which is characterized by systemic infection resulting in polyserositis, meningitis, and arthritis. Investigation of this animal disease is complicated by the enormous differences in the severity of disease caused by H. parasuis strains, ranging from lethal systemic disease to subclinical carriage. To identify differences in genotype that could account for virulence phenotypes, we established the virulence of, and performed whole genome sequence analysis on, 11 H. parasuis strains. Virulence was assessed by evaluating morbidity and mortality following intranasal challenge of Caesarean-derived, colostrum-deprived (CDCD) pigs. Genomic DNA from strains Nagasaki (serotype 5), 12939 (serotype 1), SW140 (serotype 2), 29755 (serotype 5), MN-H (serotype 13), 84-15995 (serotype 15), SW114 (serotype 3), H465 (serotype 11), D74 (serotype 9), and 174 (serotype 7) was used to generate Illumina paired-end libraries for genomic sequencing and de novo assembly. H. parasuis strains Nagasaki, 12939, SH0165 (serotype 5), SW140, 29755, and MN-H exhibited a high level of virulence. Despite minor differences in expression of disease among these groups, all pigs challenged with these strains developed clinical signs consistent with Glässer's disease between 1-7 days post-challenge. H. parasuis strains 84-15995 and SW114 were moderately virulent, in that approximately half of the pigs infected with each developed Glässer's disease. H. parasuis strains H465, D74, and 174 were minimally virulent or avirulent in the CDCD pig model. Comparative genomic analysis among strains identified several noteworthy differences in coding regions. These coding regions include predicted outer membrane, metabolism, and pilin or adhesin related genes, some of which likely contributed to the differences in virulence and systemic disease observed following challenge. These data will be useful for identifying H. parasuis virulence factors and vaccine targets.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0103787