Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells

Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e105204
Main Authors: Nenseth, Hatice Z, Dezitter, Xavier, Tesikova, Martina, Mueller, Florian, Klokk, Tove I, Hager, Gordon L, Saatcioglu, Fahri
Format: Article
Language:English
Subjects:
Androgens
Binding sites
Biology and life sciences
Blotting, Western
Cancer
Cell Line
Chromatin Immunoprecipitation
Comparative analysis
Deoxyribonucleic acid
DNA
DNA methylation
Endocrinology
Fluorescence Recovery After Photobleaching
Gene expression
Genomes
Glucocorticoid receptors
Glucocorticoids
Humans
Kinetics
Medical research
Phosphorylation
Proteins
Receptors
Receptors, Androgen - genetics
Receptors, Androgen - metabolism
Receptors, Glucocorticoid - genetics
Receptors, Glucocorticoid - metabolism
Recovery (Medical)
Regulatory sequences
Response Elements - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA polymerase
Steroid hormone receptors
Transcription factors
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Summary:Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0105204