Pyridostigmine restores cardiac autonomic balance after small myocardial infarction in mice

The effect of pyridostigmine (PYR)--an acetylcholinesterase inhibitor--on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recovery...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e104476-e104476
Main Authors: Durand, Marina T, Becari, Christiane, de Oliveira, Mauro, do Carmo, Jussara M, Silva, Carlos Alberto Aguiar, Prado, Cibele M, Fazan, Jr, Rubens, Salgado, Helio C
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholinesterase
Anesthesia
Animals
Atropine
Autonomic nervous system
Biology and Life Sciences
Blood Pressure
Cardiotonic Agents - administration & dosage
Cardiovascular disease
Cardiovascular system
Carotid artery
Cholinesterase Inhibitors - administration & dosage
Coronary artery
Coronary vessels
Drug Evaluation, Preclinical
Heart attack
Heart attacks
Heart diseases
Heart failure
Heart rate
Hemodynamics
Implantation
Infarction
Infusions, Subcutaneous
Isoflurane
Male
Medicine
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Myocardial infarction
Myocardial Infarction - drug therapy
Myocardial Infarction - physiopathology
Myocardium - pathology
Parasympathetic nervous system
Pharmacology
Phosphorylation
Physiology
Propranolol
Pyridostigmine
Pyridostigmine Bromide - administration & dosage
Rodents
Surgery
Sympathetic nervous system
Tachycardia
Telemetry
Transmitters
Vagus nerve
Ventricular Function, Left - drug effects
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Summary:The effect of pyridostigmine (PYR)--an acetylcholinesterase inhibitor--on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recovery from the surgery, basal arterial pressure and heart rate were recorded, while parasympathetic and sympathetic tone (ΔHR) was evaluated by means of methyl atropine and propranolol. After the basal hemodynamic recording the mice were subjected to left coronary artery ligation for producing myocardial infarction (MI), or sham operation, and implantation of minipumps filled with PYR or saline. Separate groups of anesthetized (isoflurane) mice previously (4 weeks) subjected to MI, or sham coronary artery ligation, were submitted to cardiac function examination. The mice exhibited an infarct length of approximately 12%, no change in arterial pressure and increased heart rate only in the 1st week after MI. Vagal tone decreased in the 1st week, while the sympathetic tone was increased in the 1st and 4th week after MI. PYR prevented the increase in heart rate but did not affect the arterial pressure. Moreover, PYR prevented the increase in sympathetic tone throughout the 4 weeks. Concerning the parasympathetic tone, PYR not only impaired its attenuation in the 1st week, but enhanced it in the 4th week. MI decreased ejection fraction and increased diastolic and systolic volume. Therefore, the pharmacological increase of peripheral acetylcholine availability by means of PYR prevented tachycardia, increased parasympathetic and decreased sympathetic tone after MI in mice.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0104476