Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise

Lipolysis involves the sequential breakdown of fatty acids from triacylglycerol and is increased during energy stress such as exercise. Adipose triglyceride lipase (ATGL) is a key regulator of skeletal muscle lipolysis and perilipin (PLIN) 5 is postulated to be an important regulator of ATGL action...

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Bibliographic Details
Published in:PloS one 2014-07, Vol.9 (7), p.e103062-e103062
Main Authors: Mason, Rachael R, Meex, Ruth C R, Russell, Aaron P, Canny, Benedict J, Watt, Matthew J
Format: Article
Language:English
Subjects:
1-Acylglycerol-3-Phosphate O-Acyltransferase - analysis
1-Acylglycerol-3-Phosphate O-Acyltransferase - metabolism
Adipocytes
Adult
Biology
Biology and Life Sciences
Cells, Cultured
Endocrinology
Exercise
Exercise - physiology
Fatty acids
Gene expression
Humans
Identification
Intracellular Signaling Peptides and Proteins - analysis
Intracellular Signaling Peptides and Proteins - metabolism
Kinases
Laboratories
Lipase
Lipase - analysis
Lipase - metabolism
Lipids
Lipolysis
Localization
Medicine and Health Sciences
Metabolism
Mitochondria
Mitochondrial DNA
Muscle Fibers, Skeletal - chemistry
Muscle Fibers, Skeletal - physiology
Muscle Fibers, Skeletal - ultrastructure
Muscle Proteins - analysis
Muscle Proteins - metabolism
Muscle, Skeletal - physiology
Muscle, Skeletal - ultrastructure
Muscles
Musculoskeletal system
Myocytes
Nutrition
Perilipin-5
Pharmacology
Phosphorylation
Physical fitness
Physiology
Post-translation
Proteins
Regulators
Research and Analysis Methods
Rest - physiology
Skeletal muscle
Studies
Triglycerides
Workloads
Young Adult
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Summary:Lipolysis involves the sequential breakdown of fatty acids from triacylglycerol and is increased during energy stress such as exercise. Adipose triglyceride lipase (ATGL) is a key regulator of skeletal muscle lipolysis and perilipin (PLIN) 5 is postulated to be an important regulator of ATGL action of muscle lipolysis. Hence, we hypothesized that non-genomic regulation such as cellular localization and the interaction of these key proteins modulate muscle lipolysis during exercise. PLIN5, ATGL and CGI-58 were highly (>60%) colocated with Oil Red O (ORO) stained lipid droplets. PLIN5 was significantly colocated with ATGL, mitochondria and CGI-58, indicating a close association between the key lipolytic effectors in resting skeletal muscle. The colocation of the lipolytic proteins, their independent association with ORO and the PLIN5/ORO colocation were not altered after 60 min of moderate intensity exercise. Further experiments in cultured human myocytes showed that PLIN5 colocation with ORO or mitochondria is unaffected by pharmacological activation of lipolytic pathways. Together, these data suggest that the major lipolytic proteins are highly expressed at the lipid droplet and colocate in resting skeletal muscle, that their localization and interactions appear to remain unchanged during prolonged exercise, and, accordingly, that other post-translational mechanisms are likely regulators of skeletal muscle lipolysis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0103062