Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal-...

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Bibliographic Details
Published in:PloS one 2014-07, Vol.9 (7), p.e100380-e100380
Main Authors: Han, Xiao-Qiang, Yue, Gar-Lee, Yue, Rui-Qi, Dong, Cai-Xia, Chan, Chung-Lap, Ko, Chun-Hay, Cheung, Wing-Shing, Luo, Ke-Wang, Dai, Hui, Wong, Chun-Kwok, Leung, Ping-Chung, Han, Quan-Bin
Format: Article
Language:English
Subjects:
Acanthopanax
Animals
B cells
B-Lymphocytes - drug effects
Beta glucan
Biology and Life Sciences
Cancer
Cell Line
Cell proliferation
Cell Proliferation - drug effects
Cytokines
Dendritic cells
Dendritic Cells - drug effects
Fluorescence
Fruit bodies
Fruiting Bodies, Fungal - chemistry
Fruits
Ganoderma
Ganoderma - chemistry
Glucan
Glucans - chemistry
Glucans - pharmacology
Humans
Immunology
Immunomodulation
Immunotherapy
Laboratories
Leukocytes (mononuclear)
Leukocytes, Mononuclear - drug effects
Lymphocytes B
Medicine
Medicine and Health Sciences
Mice
Nitric oxide
Peripheral blood mononuclear cells
Physical Sciences
Pleurotus florida
Splenocytes
Transcription factors
Tumor necrosis factor-TNF
β-Glucan
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Summary:A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0100380