Altered expression of circulating microRNA in plasma of patients with primary osteoarthritis and in silico analysis of their pathways

To analyze a set of circulating microRNA (miRNA) in plasma from patients with primary Osteoarthritis (OA) and describe the biological significance of altered miRNA in OA based on an in silico analysis of their target genes. miRNA expression was analyzed using TaqMan Low Density Arrays and independen...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-06, Vol.9 (6), p.e97690-e97690
Main Authors: Borgonio Cuadra, Verónica M, González-Huerta, Norma Celia, Romero-Córdoba, Sandra, Hidalgo-Miranda, Alfredo, Miranda-Duarte, Antonio
Format: Article
Language:English
Subjects:
Adult
Aged
Analysis
Apoptosis
Biocompatibility
Biology and life sciences
Biomarkers
Bone morphogenetic proteins
Cartilage
Cartilage diseases
Case-Control Studies
Chondrocytes
Chondrogenesis
Cluster Analysis
Development and progression
DNA methylation
Epigenetics
Female
Fibroblast growth factor receptor 1
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genes
Genetics
Humans
Injuries
Interleukin 1
Knee
Male
Medicine and Health Sciences
Metabolic pathways
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
miRNA
mRNA
Nerve growth factor
Osteoarthritis
Osteoarthritis - diagnosis
Osteoarthritis - genetics
Osteoarthritis - metabolism
Pain
Patients
Physical Sciences
Plasma
Platelet-derived growth factor
Prostate cancer
Proteins
Reproducibility of Results
Rheumatoid arthritis
Ribonucleic acid
RNA
RNA Interference
RNA polymerase
RNA, Messenger - genetics
Severity of Illness Index
Signal Transduction
Signaling
Vascular endothelial growth factor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To analyze a set of circulating microRNA (miRNA) in plasma from patients with primary Osteoarthritis (OA) and describe the biological significance of altered miRNA in OA based on an in silico analysis of their target genes. miRNA expression was analyzed using TaqMan Low Density Arrays and independent assays. The search for potential messenger RNA (mRNA) targets of the differentially expressed miRNA was performed by means of the miRWalk and miRecords database; we conducted the biological relevance of the predicted miRNA targets by pathway analysis with the Reactome and DAVID databases. We measured the expression of 380 miRNA in OA; 12 miRNA were overexpressed under the OA condition (p value, ≤0.05; fold change, >2). These results were validated by the detection of some selected miRNA by quantitative PCR (qPCR). In silico analysis showed that target messenger RNA (mRNA) were potentially regulated by these miRNA, including genes such as SMAD1, IL-1B, COL3A, VEGFA, and FGFR1, important in chondrocyte maintenance and differentiation. Some metabolic pathways affected by the miRNA: mRNA ratio are signaling Bone morphogenetic proteins (BMP), Platelet-derived growth factor (PDGF), and Nerve growth factor (NGF), these latter two involved in the process of pain. We identified 12 miRNA in the plasma of patients with primary OA. Specific miRNA that are altered in the disease could be released into plasma, either due to cartilage damage or to an inherent cellular mechanism. Several miRNA could regulate genes and pathways related with development of the disease; eight of these circulating miRNA are described, to our knowledge, for first time in OA.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0097690