Effects of postmortem interval on mouse ovary oocyte survival and maturation

To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) o...

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Bibliographic Details
Published in:PloS one 2014-05, Vol.9 (5), p.e98384-e98384
Main Authors: Zhang, Guang-Li, Ma, Jun-Yu, Sun, Quan, Hu, Meng-Wen, Yang, Xiu-Yan, Gao, Si-Hua, Jiang, Guang-Jian
Format: Article
Language:English
Subjects:
Animals
Autopsy
Biochemistry
Biology and Life Sciences
Body temperature
Carbon dioxide
Carcasses
Cell Differentiation
Cell Survival
Cooling
Diabetes
Embryos
Emissions control
Female
Forensic science
Glutathione
In vitro fertilization
Laboratories
Maturation
Medicine
Meiosis
Mice
Mitochondria
Mitochondria - metabolism
Molecular biology
Oocytes
Oocytes - cytology
Oocytes - metabolism
Ovarian cancer
Ovary - cytology
Postpartum Period
Preservation
Preservation, Biological
Spindle Apparatus - metabolism
Survival
Temperature
Temperature dependence
Temperature effects
Temperature rise
Time Factors
Zoology
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Summary:To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0098384