Anti-inflammatory activity of N-butanol extract from Ipomoea stolonifera in vivo and in vitro

Ipomoea stolonifera (I. stolonifera) has been used for the treatment of inflammatory diseases including rheumatism and rheumatoid arthritis in Chinese traditional medicine. However, the anti-inflammatory activity of I. stolonifera has not been elucidated. For this reason, the anti-inflammatory activ...

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Bibliographic Details
Published in:PloS one 2014-04, Vol.9 (4), p.e95931
Main Authors: Cai, Congyi, Chen, Yicun, Zhong, Shuping, Ji, Bin, Wang, Jinzhi, Bai, Xueting, Shi, Ganggang
Format: Article
Language:English
Subjects:
1-Butanol - chemistry
Analgesics
Analysis
Animal models
Animals
Anti-inflammatory agents
Anti-Inflammatory Agents - therapeutic use
Arthritis
Bioengineering
Biology and Life Sciences
Butanol
Care and treatment
Carrageenan
Carrageenan - toxicity
Carrageenin
Cell Line
Cell migration
Cell Survival - drug effects
Chronic illnesses
Cotton
Cytokines
Diagnosis
Edema
Edema - chemically induced
Edema - drug therapy
Exudates
Exudation
Granuloma
Health aspects
Inflammation
Inflammatory diseases
Interleukin
Interleukin 6
Ipomoea
Ipomoea - chemistry
Ipomoea pes-caprae
Ipomoea stolonifera
Laboratory animals
Lipopolysaccharides
Macrophages
Male
Malondialdehyde
Medical treatment
Medicine
Medicine and Health Sciences
Mice
Migration
Nitric oxide
Nonsteroidal anti-inflammatory drugs
Oral administration
Peroxidase
Pharmacology
Pharmacy
Physical Sciences
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Pleurisy
Prostaglandin E2
Research and Analysis Methods
Rheumatoid arthritis
Rheumatoid factor
Risk factors
Rodents
Signal transduction
Studies
Superoxide dismutase
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:Ipomoea stolonifera (I. stolonifera) has been used for the treatment of inflammatory diseases including rheumatism and rheumatoid arthritis in Chinese traditional medicine. However, the anti-inflammatory activity of I. stolonifera has not been elucidated. For this reason, the anti-inflammatory activity of n-butanol extract of I. stolonifera (BE-IS) was evaluated in vivo by using acute models (croton oil-induced mouse ear edema, carrageenan-induced rat paw edema, and carrageenan-induced rat pleurisy) and chronic models (cotton pellet-induced rat granuloma, and complete Freund's adjuvant (CFA)-induced rat arthritis). Results indicated that oral administration of BE-IS significantly attenuated croton oil-induced ear edema, decreased carrageenan-induced paw edema, reduced carrageenan-induced exudates and cellular migration, inhibited cotton pellet-induced granuloma formation and improved CFA-induced arthritis. Preliminary mechanism studies demonstrated that BE-IS decreased the levels of myeloperoxidase (MPO) and malondialdehyde (MDA), increased the activity of anti-oxidant enzyme superoxide dismutase (SOD) in vivo, and reduced the production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in lipopolysaccharide-activated RAW264.7 macrophages in vitro. Results obtained in vivo and in vitro demonstrate that BE-IS has considerable anti-inflammatory potential, which provided experimental evidences for the traditional application of Ipomoea stolonifera in inflammatory diseases.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0095931