Glycogen synthase isoforms in Synechocystis sp. PCC6803: identification of different roles to produce glycogen by targeted mutagenesis

Synechocystis sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of Synechocystis sp. PCC6803 is glycogen. While mo...

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Bibliographic Details
Published in:PloS one 2014-03, Vol.9 (3), p.e91524
Main Authors: Yoo, Sang-Ho, Lee, Byung-Hoo, Moon, Youyoun, Spalding, Martin H, Jane, Jay-Lin
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Analysis
Bacteria
Biological evolution
Biology and Life Sciences
Biosynthesis
Carbohydrates
Chains
Chlamydomonas
Cyanobacteria
Deoxyribonucleic acid
DNA
DNA sequencing
Elongation
Enzyme Activation
Enzymes
Food science
Gene Deletion
Glucose
Glycogen
Glycogen - biosynthesis
Glycogen branching enzyme
Glycogen synthase
Glycogen Synthase - genetics
Glycogen Synthase - metabolism
Glycogen synthesis
Growth rate
Homologous recombination
Homology
Isoenzymes
Isoforms
Metabolism
Mutagenesis
Mutagenesis, Site-Directed
Mutants
Mutation
Nitrogen
Nucleotide sequence
Photosynthesis
Plant sciences
Potatoes
Prokaryotes
Site-directed mutagenesis
Synechocystis
Synechocystis - genetics
Synechocystis - metabolism
Targeted mutagenesis
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Summary:Synechocystis sp. PCC6803 belongs to cyanobacteria which carry out photosynthesis and has recently become of interest due to the evolutionary link between bacteria and plant species. Similar to other bacteria, the primary carbohydrate storage source of Synechocystis sp. PCC6803 is glycogen. While most bacteria are not known to have any isoforms of glycogen synthase, analysis of the genomic DNA sequence of Synechocystis sp. PCC6803 predicts that this strain encodes two isoforms of glycogen synthase (GS) for synthesizing glycogen structure. To examine the functions of the putative GS genes, each gene (sll1393 or sll0945) was disrupted by double cross-over homologous recombination. Zymogram analysis of the two GS disruption mutants allowed the identification of a protein band corresponding to each GS isoform. Results showed that two GS isoforms (GSI and GSII) are present in Synechocystis sp. PCC6803, and both are involved in glycogen biosynthesis with different elongation properties: GSI is processive and GSII is distributive. Total GS activities in the mutant strains were not affected and were compensated by the remaining isoform. Analysis of the branch-structure of glycogen revealed that the sll1393- mutant (GSI-) produced glycogen containing more intermediate-length chains (DP 8-18) at the expense of shorter and longer chains compared with the wild-type strain. The sll0945- mutant (GSII-) produced glycogen similar to the wild-type, with only a slightly higher proportion of short chains (DP 4-11). The current study suggests that GS isoforms in Synechocystis sp. PCC6803 have different elongation specificities in the biosynthesis of glycogen, combined with ADP-glucose pyrophosphorylase and glycogen branching enzyme.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0091524