Single cell genomics: advances and future perspectives

Advances in whole-genome and whole-transcriptome amplification have permitted the sequencing of the minute amounts of DNA and RNA present in a single cell, offering a window into the extent and nature of genomic and transcriptomic heterogeneity which occurs in both normal development and disease. Si...

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Bibliographic Details
Published in:PLoS genetics 2014-01, Vol.10 (1), p.e1004126-e1004126
Main Authors: Macaulay, Iain C, Voet, Thierry
Format: Article
Language:English
Subjects:
Analysis
Biology
Cell division
Computational Biology - methods
Deoxyribonucleic acid
DNA
DNA methylation
Epigenomics
Forecasts and trends
Gene expression
Genetic aspects
Genetic Heterogeneity
Genetic Variation
Genomes
Genomics
Genomics - methods
Humans
Mutation
Phylogenetics
Population
Review
Sequence Analysis, DNA
Single-Cell Analysis - methods
Transcriptome
Unicellular organisms
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Summary:Advances in whole-genome and whole-transcriptome amplification have permitted the sequencing of the minute amounts of DNA and RNA present in a single cell, offering a window into the extent and nature of genomic and transcriptomic heterogeneity which occurs in both normal development and disease. Single-cell approaches stand poised to revolutionise our capacity to understand the scale of genomic, epigenomic, and transcriptomic diversity that occurs during the lifetime of an individual organism. Here, we review the major technological and biological breakthroughs achieved, describe the remaining challenges to overcome, and provide a glimpse into the promise of recent and future developments.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1004126