Understanding the role of growth factors in modulating stem cell tenogenesis

Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a succ...

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Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e83734-e83734
Main Authors: Gonçalves, Ana I, Rodrigues, Márcia T, Lee, Sang-Jin, Atala, Anthony, Yoo, James J, Reis, Rui L, Gomes, Manuela E
Format: Article
Language:English
Subjects:
Amniotic fluid
Amniotic Fluid - cytology
Basic Helix-Loop-Helix Transcription Factors - metabolism
Biology
Biomechanics
Collagen
Collagen (type I)
Collagen Type I - metabolism
Collagen Type III - metabolism
Cytology
Cytoskeleton - metabolism
Decorin
Decorin - metabolism
Extracellular matrix
Extracellular Matrix - metabolism
Extracellular Matrix Proteins - metabolism
Female
Fibroblast growth factor 2
Fibroblasts
Gene expression
Genes
Growth factors
Hand surgery
Humans
Intercellular Signaling Peptides and Proteins - metabolism
Laboratories
Ligaments
Medicine
Platelet-derived growth factor
Platelet-derived growth factor BB
Polymers
Protein Transport
Proteins
Regeneration
Social aspects
Social impact
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Surgery
Tenascin
Tenascin - metabolism
Tenascin C
Tendon Injuries - metabolism
Tendon Injuries - therapy
Tendons
Tendons - cytology
Tendons - physiology
Tissue engineering
Transforming growth factor-b1
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Summary:Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, their exogenous addition can further stimulate stem cells into the tenogenic lineage, which might eventually depend on stem cells source. In the present study we investigate the tenogenic differentiation potential of human- amniotic fluid stem cells (hAFSCs) and adipose-derived stem cells (hASCs) with several GFs associated to tendon development and healing; namely, EGF, bFGF, PDGF-BB and TGF-β1. Stem cells response to biochemical stimuli was studied by screening of tendon-related genes (collagen type I, III, decorin, tenascin C and scleraxis) and proteins found in tendon extracellular matrix (ECM) (Collagen I, III, and Tenascin C). Despite the fact that GFs did not seem to influence the synthesis of tendon ECM proteins, EGF and bFGF influenced the expression of tendon-related genes in hAFSCs, while EGF and PDGF-BB stimulated the genetic expression in hASCs. Overall results on cellular alignment morphology, immunolocalization and PCR analysis indicated that both stem cell source can be biochemically induced towards tenogenic commitment, validating the potential of hASCs and hAFSCs for tendon regeneration strategies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0083734