Microarray analysis of tomato's early and late wound response reveals new regulatory targets for Leucine aminopeptidase A

Wounding due to mechanical injury or insect feeding causes a wide array of damage to plant cells including cell disruption, desiccation, metabolite oxidation, and disruption of primary metabolism. In response, plants regulate a variety of genes and metabolic pathways to cope with injury. Tomato (Sol...

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Bibliographic Details
Published in:PloS one 2013-10, Vol.8 (10), p.e77889-e77889
Main Authors: Scranton, Melissa A, Fowler, Jonathan H, Girke, Thomas, Walling, Linda L
Format: Article
Language:English
Subjects:
Aminopeptidase
Arabidopsis
Bioinformatics
Biology
Biomarkers - metabolism
Blotting, Western
Cell disruption
Dehydrin
Desiccation
DNA microarrays
Drying
Experiments
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes
Genomics
Herbivores
Injuries
Insects
Leaves
Leucine
Leucyl Aminopeptidase - genetics
Leucyl Aminopeptidase - metabolism
Lycopersicon esculentum - genetics
Lycopersicon esculentum - growth & development
Lycopersicon esculentum - metabolism
Metabolic pathways
Metabolism
Oligonucleotide Array Sequence Analysis
Oxidation
Pathogenesis
Pathways
Plant cells
Plant sciences
Potatoes
Proteins
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Plant - genetics
Signal transduction
Signaling
Solanaceae
Solanum lycopersicum
Studies
Tomatoes
VOCs
Volatile organic compounds
Wound Healing
Wounding
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Summary:Wounding due to mechanical injury or insect feeding causes a wide array of damage to plant cells including cell disruption, desiccation, metabolite oxidation, and disruption of primary metabolism. In response, plants regulate a variety of genes and metabolic pathways to cope with injury. Tomato (Solanum lycopersicum) is a model for wound signaling but few studies have examined the comprehensive gene expression profiles in response to injury. A cross-species microarray approach using the TIGR potato 10-K cDNA array was analyzed for large-scale temporal (early and late) and spatial (locally and systemically) responses to mechanical wounding in tomato leaves. These analyses demonstrated that tomato regulates many primary and secondary metabolic pathways and this regulation is dependent on both timing and location. To determine if LAP-A, a known modulator of wound signaling, influences gene expression beyond the core of late wound-response genes, changes in RNAs from healthy and wounded Leucine aminopeptidase A-silenced (LapA-SI) and wild-type (WT) leaves were examined. While most of the changes in gene expression after wounding in LapA-SI leaves were similar to WT, overall responses were delayed in the LapA-SI leaves. Moreover, two pathogenesis-related 1 (PR-1c and PR-1a2) and two dehydrin (TAS14 and Dhn3) genes were negatively regulated by LAP-A. Collectively, this study has shown that tomato wound responses are complex and that LAP-A's role in modulation of wound responses extends beyond the well described late-wound gene core.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0077889