Effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos

Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the t...

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Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e72302-e72302
Main Authors: Peñagaricano, Francisco, Souza, Alex H, Carvalho, Paulo D, Driver, Ashley M, Gambra, Rocio, Kropp, Jenna, Hackbart, Katherine S, Luchini, Daniel, Shaver, Randy D, Wiltbank, Milo C, Khatib, Hasan
Format: Article
Language:English
Subjects:
Agriculture
Amino acids
Animal lactation
Animal sciences
Animals
Base Sequence
Biology
Blastocyst
Blastocysts
Bovidae
Cattle
Dairy industry
Deoxyribonucleic acid
Diet
Dietary Supplements
DNA
DNA methylation
DNA Primers
Embryogenesis
Embryonic development
Embryonic growth stage
Embryos
Epigenetics
Female
Gene expression
Gene Expression Regulation, Developmental
Gene sequencing
Gene set enrichment analysis
Genes
Genomics
Human nutrition
Immune response
Immune system
Immunology
Metabolism
Methionine
Methionine - administration & dosage
Methylation
Nutrition
Nutrition research
Offspring
Pregnancy
Ribonucleic acid
RNA
RNA sequencing
Rodents
Studies
Transcription
Transcription (Genetics)
Transcriptome
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Summary:Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. Holstein cows were randomly assigned to one of two treatments differing in level of dietary methionine (1.89 Met vs. 2.43 Met % of metabolizable protein) from calving until embryo flushing. High quality preimplantation embryos from individual cows were pooled and then analyzed by RNA sequencing. Remarkably, a subtle difference in methionine supplementation in maternal diet was sufficient to cause significant changes in the transcriptome of the embryos. A total of 276 genes out of 10,662 showed differential expression between treatments (FDR
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0072302