Evaluation of injectable constructs for bone repair with a subperiosteal cranial model in the rat

While testing regenerative medicine strategies, the use of animal models that match the research questions and that are related to clinical translation is crucial. During the initial stage of evaluating new strategies for bone repair, the main goal is to state whether the strategies efficiently indu...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e71683-e71683
Main Authors: Kisiel, Marta, Klar, Agnieszka S, Martino, Mikaël M, Ventura, Manuela, Hilborn, Jöns
Format: Article
Language:English
Subjects:
Angiogenesis
Animal model
Animal models
Animals
Augmentation
Biocompatibility
Biocompatible Materials
Biological products
Biology
Biomedical materials
Bone growth
Bone healing
Bone morphogenetic protein 2
Bone Morphogenetic Protein 2 - metabolism
Bone morphogenetic proteins
Bone Regeneration
Bone repairr matrix
Bones
Calvaria
Computed tomography
Defects
Engineering Science
Evaluation
Growth factors
Histology
Hyaluronan
Hyaluronic acid
Hyaluronic Acid - metabolism
Hydrogels
Hydrogels - metabolism
Immunohistochemistry
Kirurgi
Laboratory animals
Male
Medicine
Minimally invasive surgery
Models, Animal
Neovascularization, Physiologic
Orthopaedics
Ortopedi
Osteogenesis
Osteogenesis - physiology
Polymer chemistry
Proteins
Rats
Regeneration (physiology)
Regenerative medicine
Repair
Skull
Skull - physiology
Skull - surgery
Subperiosteal rat model
Surgery
Teknisk fysik
Tissue Engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:While testing regenerative medicine strategies, the use of animal models that match the research questions and that are related to clinical translation is crucial. During the initial stage of evaluating new strategies for bone repair, the main goal is to state whether the strategies efficiently induce the formation of new bone tissue at an orthotopic site. Here, we present a subperiosteal model in rat calvaria that allow the evaluation of a broad range of approaches including bone augmentation, replacement and regeneration. The model is a fast to perform, minimally invasive, and has clearly defined control groups. The procedure enables to evaluate the outcomes quantitatively using micro-computed tomography and qualitatively by histology and immunohistochemistry. We established this new model, using bone morphogenetic protein-2 as an osteoinductive factor and hyaluronic acid hydrogel as injectable biomaterial. We showed that this subperiosteal cranial model offers a minimally invasive and promising solution for a rapid initial evaluation of injectables for bone repair. We believe that this approach could be a powerful platform for orthopedic research and regenerative medicine.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0071683