SGO1 maintains bovine meiotic and mitotic centromeric cohesions of sister chromatids and directly affects embryo development

Shugoshin (SGO) is a critical factor that enforces cohesion from segregation of paired sister chromatids during mitosis and meiosis. It has been studied mainly in invertebrates. Knowledge of SGO(s) in a mammalian system has only been reported in the mouse and Hela cells. In this study, the functions...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e73636-e73636
Main Authors: Yin, Feng-Xia, Li, Guang-Peng, Bai, Chun-Ling, Liu, Yang, Wei, Zhu-Ying, Liang, Cheng-Guang, Bunch, Thomas D, Zan, Lin-Sen
Format: Article
Language:English
Subjects:
Animal sciences
Animals
Apoptosis
Base Sequence
Biology
Biotechnology
Breast cancer
Cattle
Cell cycle
Cell Cycle Proteins - genetics
Cell Cycle Proteins - physiology
Cell division
Cells, Cultured
Centromere
Chromatids
Chromosomes
Cohesion
Colorectal cancer
Defects
Depletion
Dissociation
DNA Primers
Education
Embryo fibroblasts
Embryogenesis
Embryonic development
Embryonic Development - physiology
Embryonic growth stage
Fibroblasts
Gene expression
Homology
Invertebrates
Kinases
Laboratory animals
Mediation
Meiosis
Metaphase
Microscopy, Fluorescence
Mitosis
Oocytes
Overexpression
Poles
Proteins
RNA, Small Interfering - genetics
Separation
siRNA
Sister chromatids
Studies
Tumorigenesis
Yeast
Zoology
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Summary:Shugoshin (SGO) is a critical factor that enforces cohesion from segregation of paired sister chromatids during mitosis and meiosis. It has been studied mainly in invertebrates. Knowledge of SGO(s) in a mammalian system has only been reported in the mouse and Hela cells. In this study, the functions of SGO1 in bovine oocytes during meiotic maturation, early embryonic development and somatic cell mitosis were investigated. The results showed that SGO1 was expressed from germinal vesicle (GV) to the metaphase II stage. SGO1 accumulated on condensed and scattered chromosomes from pre-metaphase I to metaphase II. The over-expression of SGO1 did not interfere with the process of homologous chromosome separation, although once separated they were unable to move to the opposing spindle poles. This often resulted in the formation of oocytes with 60 replicated chromosomes. Depletion of SGO1 in GV oocytes affected chromosomal separation resulting in abnormal chromosome alignment at a significantly higher proportion than in control oocytes. Knockdown of SGO1 expression significantly decreased the embryonic developmental rate and quality. To further confirm the function(s) of SGO1 during mitosis, bovine embryonic fibroblast cells were transfected with SGO1 siRNAs. SGO1 depletion induced the premature dissociation of chromosomal cohesion at the centromere and along the chromosome arm giving rise to abnormal appearing mitotic patterns. The results of this study infer that SGO1 is involved in the centromeric cohesion of sister chromatids and chromosomal movement towards the spindle poles. Depletion of SGO1 causes arrestment of cell division in meiosis and mitosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0073636