Novel polyomaviruses of nonhuman primates: genetic and serological predictors for the existence of multiple unknown polyomaviruses within the human population

Polyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human po...

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Bibliographic Details
Published in:PLoS pathogens 2013-06, Vol.9 (6), p.e1003429-e1003429
Main Authors: Scuda, Nelly, Madinda, Nadege Freda, Akoua-Koffi, Chantal, Adjogoua, Edgard Valerie, Wevers, Diana, Hofmann, Jörg, Cameron, Kenneth N, Leendertz, Siv Aina J, Couacy-Hymann, Emmanuel, Robbins, Martha, Boesch, Christophe, Jarvis, Michael A, Moens, Ugo, Mugisha, Lawrence, Calvignac-Spencer, Sébastien, Leendertz, Fabian H, Ehlers, Bernhard
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Basale medisinske, odontologiske og veterinærmedisinske fag: 710
Basic medical, dental and veterinary science disciplines: 710
Biology
Capsid Proteins - blood
Capsid Proteins - genetics
Enzyme-Linked Immunosorbent Assay
Female
Genetic aspects
Health aspects
Host-parasite relationships
Humans
Male
Medical disciplines: 700
Medical genetics: 714
Medicine
Medisinsk genetikk: 714
Medisinske Fag: 700
Microbiology
Monkey Diseases - blood
Monkey Diseases - genetics
Monkeys & apes
National parks
Phylogeny
Physiological aspects
Platyrrhini - blood
Platyrrhini - virology
Polyoma virus
Polyomavirus - genetics
Polyomavirus - metabolism
Polyomavirus Infections - blood
Polyomavirus Infections - genetics
Population genetics
Primates
VDP
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Summary:Polyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human polyomaviruses, we have utilized a combinatorial approach comprised of initial degenerate primer-based PCR identification and phylogenetic analysis of nonhuman primate (NHP) polyomavirus species, followed by polyomavirus-specific serological analysis of human sera. Using this approach we identified twenty novel NHP polyomaviruses: nine in great apes (six in chimpanzees, two in gorillas and one in orangutan), five in Old World monkeys and six in New World monkeys. Phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. To determine whether the remaining chimpanzee polyomaviruses had potential human counterparts, the major viral capsid proteins (VP1) of four chimpanzee polyomaviruses were expressed in E. coli for use as antigens in enzyme-linked immunoassay (ELISA). Human serum/plasma samples from both Côte d'Ivoire and Germany showed frequent seropositivity for the four viruses. Antibody pre-adsorption-based ELISA excluded the possibility that reactivities resulted from binding to known human polyomaviruses. Together, these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1003429