Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II

Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromos...

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Bibliographic Details
Published in:PLoS genetics 2013-06, Vol.9 (6), p.e1003579-e1003579
Main Authors: Venkova-Canova, Tatiana, Baek, Jong Hwan, Fitzgerald, Peter C, Blokesch, Melanie, Chattoraj, Dhruba K
Format: Article
Language:English
Subjects:
Bacterial genetics
Biology
Cholera - genetics
Cholera - microbiology
Chromosome Segregation - genetics
Chromosomes
Chromosomes, Bacterial - genetics
DNA Helicases
DNA Replication - genetics
DNA-Binding Proteins - genetics
Genetic aspects
Humans
Medical research
Microbiology
Physiological aspects
Plasmids
Proteins
Trans-Activators
Vibrio cholerae
Vibrio cholerae - genetics
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Summary:Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly, without nucleation at the centromere, ParB2 could also bind directly to a related 39-mer. The 39-mers are the strongest inhibitors of chromosome II replication and they mediate inhibition by binding the replication initiator protein. ParB2 thus appears to promote replication by out-competing initiator binding to the 39-mers using two mechanisms: spreading into one and direct binding to the other. We suggest that both these are novel mechanisms to coordinate replication initiation with segregation of chromosomes.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1003579