Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis

Reactive oxygen species (ROS) are produced in plant cells in response to diverse biotic and abiotic stresses as well as during normal growth and development. Although a large number of transcription factor (TF) genes are up- or down-regulated by ROS, currently very little is known about the function...

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Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e70289-e70289
Main Authors: Sewelam, Nasser, Kazan, Kemal, Thomas-Hall, Skye R, Kidd, Brendan N, Manners, John M, Schenk, Peer M
Format: Article
Language:English
Subjects:
Abiotic stress
Agriculture
Analysis
Antioxidants
Antioxidants (Nutrients)
Apoptosis
Arabidopsis
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biology
Biosynthesis
Calcium
Calcium signalling
Catalase - genetics
Catalase - metabolism
Enzymes
Ethylene
Food
Gene expression
Genes
Genetic aspects
Hormones
Hydrogen peroxide
Industrial research
Kinases
Laboratories
NAD(P)H oxidase
NADPH Oxidases - metabolism
Oxidases
Oxidative Stress
Oxygen
Phosphoric Monoester Hydrolases - genetics
Phosphoric Monoester Hydrolases - metabolism
Physiology
Plant biology
Plant cells
Plant growth
Plant resistance
Plants (botany)
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Signal transduction
Transcription (Genetics)
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Reactive oxygen species (ROS) are produced in plant cells in response to diverse biotic and abiotic stresses as well as during normal growth and development. Although a large number of transcription factor (TF) genes are up- or down-regulated by ROS, currently very little is known about the functions of these TFs during oxidative stress. In this work, we examined the role of ERF6 (ETHYLENE RESPONSE FACTOR6), an AP2/ERF domain-containing TF, during oxidative stress responses in Arabidopsis. Mutant analyses showed that NADPH oxidase (RbohD) and calcium signaling are required for ROS-responsive expression of ERF6. erf6 insertion mutant plants showed reduced growth and increased H2O2 and anthocyanin levels. Expression analyses of selected ROS-responsive genes during oxidative stress identified several differentially expressed genes in the erf6 mutant. In particular, a number of ROS responsive genes, such as ZAT12, HSFs, WRKYs, MAPKs, RBOHs, DHAR1, APX4, and CAT1 were more strongly induced by H2O2 in erf6 plants than in wild-type. In contrast, MDAR3, CAT3, VTC2 and EX1 showed reduced expression levels in the erf6 mutant. Taken together, our results indicate that ERF6 plays an important role as a positive antioxidant regulator during plant growth and in response to biotic and abiotic stresses.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0070289