RNAi screen reveals potentially novel roles of cytokines in myoblast differentiation

Cytokines are cell-secreted signaling molecules that modulate various cellular functions, with the best-characterized roles in immune responses. The expression of numerous cytokines in skeletal muscle tissues and muscle cells has been reported, but their function in skeletal myogenesis, the formatio...

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Bibliographic Details
Published in:PloS one 2013-07, Vol.8 (7), p.e68068-e68068
Main Authors: Ge, Yejing, Waldemer, Rachel J, Nalluri, Ramakrishna, Nuzzi, Paul D, Chen, Jie
Format: Article
Language:English
Subjects:
Animals
Biology
Cell cycle
Cell differentiation
Cell Differentiation - genetics
Cell Line
Chemokines
Chemokines - genetics
Cytokines
Cytokines - genetics
Developmental biology
Differentiation
Health aspects
Immune response
Interleukins
Interleukins - genetics
Kinases
Mice
Muscle Development - genetics
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - metabolism
Muscles
Musculoskeletal system
Myoblasts
Myoblasts - cytology
Myoblasts - metabolism
Myogenesis
Regeneration
Regulators
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA-mediated interference
Rodents
Signal Transduction - genetics
Signaling
Skeletal muscle
Target recognition
Tissues
Tumor necrosis factor
Tumor necrosis factor-TNF
Tumor Necrosis Factors - genetics
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Summary:Cytokines are cell-secreted signaling molecules that modulate various cellular functions, with the best-characterized roles in immune responses. The expression of numerous cytokines in skeletal muscle tissues and muscle cells has been reported, but their function in skeletal myogenesis, the formation of skeletal muscle, has been largely underexplored. To systematically examine the potential roles of cytokines in skeletal myogenesis, we undertook an RNAi screen of 134 mouse cytokine genes for their involvement in the differentiation of C2C12 myoblasts. Our results have uncovered 29 cytokines as strong candidates for novel myogenic regulators, potentially conferring positive and negative regulation at distinct stages of myogenesis. These candidates represent a diverse collection of cytokine families, including interleukins, TNF-related factors, and chemokines. Our findings suggest the fundamental importance of cytokines in the cell-autonomous regulation of myoblast differentiation, and may facilitate future identification of novel therapeutic targets for improving muscle regeneration and growth in health and diseases.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0068068