Powdery mildew resistance in tomato by impairment of SlPMR4 and SlDMR1

Genetic dissection of disease susceptibility in Arabidopsis to powdery and downy mildew has identified multiple susceptibility (S) genes whose impairment results in disease resistance. Although several of these S-genes have been cloned and characterized in more detail it is unknown to which degree t...

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Bibliographic Details
Published in:PloS one 2013-06, Vol.8 (6), p.e67467
Main Authors: Huibers, Robin P, Loonen, Annelies E H M, Gao, Dongli, Van den Ackerveken, Guido, Visser, Richard G F, Bai, Yuling
Format: Article
Language:English
Subjects:
Agriculture
Airborne microorganisms
Alleles
Amino acids
Arabidopsis
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - microbiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Ascomycota - physiology
Barley
Base Sequence
Biology
Breeding
Conserved Sequence
Crop diseases
Disease resistance
Disease Resistance - drug effects
Disease Resistance - genetics
Disease susceptibility
Dissection
Downy mildew
Fitness
Flowers & plants
Fungi
Gene silencing
Genes
Genetic aspects
Genetic engineering
Genomics
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Homoserine - metabolism
Homoserine - pharmacology
Homoserine kinase
Humidity
Impairment
Laboratories
Lycopersicon esculentum - genetics
Lycopersicon esculentum - growth & development
Lycopersicon esculentum - microbiology
Molecular Sequence Data
Mutagenesis
Mutation
Pathogens
Phosphotransferases (Alcohol Group Acceptor) - genetics
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Plant breeding
Plant diseases
Plant Diseases - genetics
Plant Diseases - microbiology
Plant growth
Plant Proteins - genetics
Plant Proteins - metabolism
Powdery mildew
Reproductive fitness
RNA Interference
RNA-mediated interference
Solanum lycopersicum
Tomatoes
Wildlife conservation
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Summary:Genetic dissection of disease susceptibility in Arabidopsis to powdery and downy mildew has identified multiple susceptibility (S) genes whose impairment results in disease resistance. Although several of these S-genes have been cloned and characterized in more detail it is unknown to which degree their function in disease susceptibility is conserved among different plant species. Moreover, it is unclear whether impairment of such genes has potential in disease resistance breeding due to possible fitness costs associated with impaired alleles. Here we show that the Arabidopsis PMR4 and DMR1, genes encoding a callose synthase and homoserine kinase respectively, have functional orthologs in tomato with respect to their S-gene function. Silencing of both genes using RNAi resulted in resistance to the tomato powdery mildew fungus Oidium neolycopersici. Resistance to O. neolycopersici by SlDMR1 silencing was associated with severely reduced plant growth whereas SlPMR4 silencing was not. SlPMR4 is therefore a suitable candidate gene as target for mutagenesis to obtain alleles that can be deployed in disease resistance breeding of tomato.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0067467