Distinct functional and temporal requirements for zebrafish Hdac1 during neural crest-derived craniofacial and peripheral neuron development

The regulation of gene expression is accomplished by both genetic and epigenetic means and is required for the precise control of the development of the neural crest. In hdac1(b382) mutants, craniofacial cartilage development is defective in two distinct ways. First, fewer hoxb3a, dlx2 and dlx3-expr...

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Bibliographic Details
Published in:PloS one 2013-05, Vol.8 (5), p.e63218-e63218
Main Authors: Ignatius, Myron S, Unal Eroglu, Arife, Malireddy, Smitha, Gallagher, Glen, Nambiar, Roopa M, Henion, Paul D
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biology
Branchial Region - abnormalities
Branchial Region - embryology
Branchial Region - pathology
Cartilage
Cell Differentiation - drug effects
Chondrocytes
Chromatin
Collagen
Craniofacial Abnormalities - embryology
Craniofacial Abnormalities - pathology
Craniofacial growth
Danio rerio
Defects
Deoxyribonucleic acid
Developmental biology
Differentiation
DNA
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - metabolism
Embryo, Nonmammalian - pathology
Embryos
Enteric nervous system
Enzymatic activity
Enzyme activity
Enzymes
Epigenetic inheritance
Face - abnormalities
Face - embryology
Face - pathology
Gene expression
Histone deacetylase
Histone Deacetylase 1 - genetics
Histone Deacetylase 1 - metabolism
Hydroxamic Acids - pharmacology
Hyoid Bone - abnormalities
Hyoid Bone - drug effects
Hyoid Bone - embryology
Hyoid Bone - pathology
Mandible
Mandible - abnormalities
Mandible - drug effects
Mandible - embryology
Mandible - pathology
Mutants
Mutation - genetics
Nervous system
Neural crest
Neural Crest - drug effects
Neural Crest - embryology
Neural Crest - metabolism
Neural Crest - pathology
Neural stem cells
Neurons
Neurons - drug effects
Neurons - metabolism
Neurons - pathology
Neurosciences
Norepinephrine
Peripheral nervous system
Peripheral Nervous System - drug effects
Peripheral Nervous System - embryology
Peripheral Nervous System - pathology
Phenotype
Skull - abnormalities
Skull - embryology
Skull - pathology
Stem Cells - drug effects
Stem Cells - metabolism
Stem Cells - pathology
Sympathetic Nervous System - drug effects
Sympathetic Nervous System - metabolism
Sympathetic Nervous System - pathology
Time Factors
Transcription factors
Zebrafish
Zebrafish - embryology
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:The regulation of gene expression is accomplished by both genetic and epigenetic means and is required for the precise control of the development of the neural crest. In hdac1(b382) mutants, craniofacial cartilage development is defective in two distinct ways. First, fewer hoxb3a, dlx2 and dlx3-expressing posterior branchial arch precursors are specified and many of those that are consequently undergo apoptosis. Second, in contrast, normal numbers of progenitors are present in the anterior mandibular and hyoid arches, but chondrocyte precursors fail to terminally differentiate. In the peripheral nervous system, there is a disruption of enteric, DRG and sympathetic neuron differentiation in hdac1(b382) mutants compared to wildtype embryos. Specifically, enteric and DRG-precursors differentiate into neurons in the anterior gut and trunk respectively, while enteric and DRG neurons are rarely present in the posterior gut and tail. Sympathetic neuron precursors are specified in hdac1(b382) mutants and they undergo generic neuronal differentiation but fail to undergo noradrenergic differentiation. Using the HDAC inhibitor TSA, we isolated enzyme activity and temporal requirements for HDAC function that reproduce hdac1(b382) defects in craniofacial and sympathetic neuron development. Our study reveals distinct functional and temporal requirements for zebrafish hdac1 during neural crest-derived craniofacial and peripheral neuron development.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0063218