Molecular characterization of the fecal microbiota in patients with nonalcoholic steatohepatitis--a longitudinal study

The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. Fecal microbiota of hist...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e62885-e62885
Main Authors: Wong, Vincent Wai-Sun, Tse, Chi-Hang, Lam, Tommy Tsan-Yuk, Wong, Grace Lai-Hung, Chim, Angel Mei-Ling, Chu, Winnie Chiu-Wing, Yeung, David Ka-Wai, Law, Patrick Tik-Wan, Kwan, Hoi-Shan, Yu, Jun, Sung, Joseph Jao-Yiu, Chan, Henry Lik-Yuen
Format: Article
Language:English
Subjects:
Abundance
Adolescent
Adult
Aged
Alcohol
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
Biology
Case-Control Studies
Correlation analysis
Diet
Discriminant analysis
Dysbacteriosis
Fatty liver
Fatty Liver - microbiology
Fecal microflora
Feces
Feces - microbiology
Female
Gastroenterology
Gastrointestinal agents
Hepatitis
Histology
Humans
Immunity
Intestinal microflora
Irritable bowel syndrome
Life sciences
Liver
Liver diseases
Longitudinal Studies
Magnetic resonance
Magnetic resonance spectroscopy
Male
Medical research
Medicine
Metabolism
Microbiota
Microbiota (Symbiotic organisms)
Middle Aged
Nuclear magnetic resonance spectroscopy
Obesity
Patients
Phylogeny
Physiological aspects
Probiotics
Ribonucleic acid
Ribosomal RNA
RNA
Rodents
rRNA 16S
Spectroscopy
Steatosis
Triglycerides
Type 2 diabetes
Young Adult
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Summary:The human gut microbiota has profound influence on host metabolism and immunity. This study characterized the fecal microbiota in patients with nonalcoholic steatohepatitis (NASH). The relationship between microbiota changes and changes in hepatic steatosis was also studied. Fecal microbiota of histology-proven NASH patients and healthy controls was analyzed by 16S ribosomal RNA pyrosequencing. NASH patients were from a previously reported randomized trial on probiotic treatment. Proton-magnetic resonance spectroscopy was performed to monitor changes in intrahepatic triglyceride content (IHTG). A total of 420,344 16S sequences with acceptable quality were obtained from 16 NASH patients and 22 controls. NASH patients had lower fecal abundance of Faecalibacterium and Anaerosporobacter but higher abundance of Parabacteroides and Allisonella. Partial least-square discriminant analysis yielded a model of 10 genera that discriminated NASH patients from controls. At month 6, 6 of 7 patients in the probiotic group and 4 of 9 patients in the usual care group had improvement in IHTG (P=0.15). Improvement in IHTG was associated with a reduction in the abundance of Firmicutes (R(2)=0.4820, P=0.0028) and increase in Bacteroidetes (R(2)=0.4366, P=0.0053). This was accompanied by corresponding changes at the class, order and genus levels. In contrast, bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment. NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis. Further studies are required to investigate the mechanism underlying the interaction between gut microbes and the liver.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0062885