Comparing HLA shared epitopes in French Caucasian patients with scleroderma

Although many studies have analyzed HLA allele frequencies in several ethnic groups in patients with scleroderma (SSc), none has been done in French Caucasian patients and none has evaluated which one of the common amino acid sequences, (67)FLEDR(71), shared by HLA-DRB susceptibility alleles, or (71...

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Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e36870-e36870
Main Authors: Azzouz, Doua F, Rak, Justyna M, Fajardy, Isabelle, Allanore, Yannick, Tiev, Kiet Phong, Farge-Bancel, Dominique, Martin, Marielle, Kanaan, Sami B, Pagni, Philippe P, Hachulla, Eric, Harlé, Jean Robert, Didelot, Rémi, Granel, Brigitte, Cabane, Jean, Roudier, Jean, Lambert, Nathalie C
Format: Article
Language:English
Subjects:
Alleles
Amino acids
Analysis
Antigen presentation
Antigenic determinants
Autoantibodies
Autoimmunity
Biology
Celiac disease
Diabetes
DNA polymerases
Drb1 protein
Epitopes
Epitopes - genetics
Epitopes - immunology
European Continental Ancestry Group - genetics
Female
Gene Frequency
Genes
Genetic Predisposition to Disease
Haplotypes
Histocompatibility antigen HLA
HLA antigens
HLA Antigens - genetics
HLA Antigens - immunology
HLA-DQ beta-Chains - genetics
HLA-DQ beta-Chains - immunology
HLA-DRB1 Chains - genetics
HLA-DRB1 Chains - immunology
HLA-DRB5 Chains - genetics
HLA-DRB5 Chains - immunology
Humans
Linkage Disequilibrium
Male
Medical research
Medicine
Middle Aged
Minority & ethnic groups
Patients
Rheumatoid arthritis
RNA
RNA polymerase
RNA, Messenger - genetics
RNA-directed DNA polymerase
Scleroderma
Scleroderma (Disease)
Scleroderma, Systemic - genetics
Scleroderma, Systemic - immunology
Subgroups
Therapeutic applications
Tissue typing
White people
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Summary:Although many studies have analyzed HLA allele frequencies in several ethnic groups in patients with scleroderma (SSc), none has been done in French Caucasian patients and none has evaluated which one of the common amino acid sequences, (67)FLEDR(71), shared by HLA-DRB susceptibility alleles, or (71)TRAELDT(77), shared by HLA-DQB1 susceptibility alleles in SSc, was the most important to develop the disease. HLA-DRB and DQB typing was performed for a total of 468 healthy controls and 282 patients with SSc allowing FLEDR and TRAELDT analyses. Results were stratified according to patient's clinical subtypes and autoantibody status. Moreover, standardized HLA-DRß1 and DRß5 reverse transcriptase Taqman PCR assays were developed to quantify ß1 and ß5 mRNA in 20 subjects with HLA-DRB1*15 and/or DRB1*11 haplotypes. FLEDR motif is highly associated with diffuse SSc (χ(2) = 28.4, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0036870