Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin β1 and BMP2 signaling cross-talk

Fluid shear stress plays an important role in bone osteogenic differentiation. It is traditionally believed that pulsed and continuous stress load is more favorable for fracture recovery and bone homeostasis. However, according to our clinical practice, we notice that one single stress load is also...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e61600-e61600
Main Authors: Mai, Zhihui, Peng, Zhuli, Wu, Sihan, Zhang, Jinglan, Chen, Lin, Liang, Huangyou, Bai, Ding, Yan, Guangmei, Ai, Hong
Format: Article
Language:English
Subjects:
3T3 Cells
Actin
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Analysis of Variance
Animals
Anthraquinones
Biochemistry
Biocompatibility
Biology
Biomedical materials
Bone marrow
Bone morphogenetic protein 2
Bone Morphogenetic Protein 2 - metabolism
Bone turnover
Cell Differentiation - physiology
Cell growth
Chemistry
Collagen
Collagen (type I)
Differentiation
Engineering
Enzyme-Linked Immunosorbent Assay
Flow system
Fluid flow
Gene expression
Gene regulation
Genes
Homeostasis
Hydrodynamics
Integrin beta1 - metabolism
Kinases
Mechanical stimuli
Mice
Microscopy, Confocal
Orthodontics
Osteoblastogenesis
Osteogenesis - physiology
Osteoid
Proteins
Real-Time Polymerase Chain Reaction
Receptor Cross-Talk - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
Secretion
Shear Strength - physiology
Shear stress
Signal Transduction - physiology
Signaling
Stem cells
Stress, Mechanical
Time Factors
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Summary:Fluid shear stress plays an important role in bone osteogenic differentiation. It is traditionally believed that pulsed and continuous stress load is more favorable for fracture recovery and bone homeostasis. However, according to our clinical practice, we notice that one single stress load is also sufficient to trigger osteogenic differentiation. In the present study, we subject osteoblast MC3T3-E1 cells to single bout short duration fluid shear stress by using a parallel plate flow system. The results show that 1 hour of fluid shear stress at 12 dyn/cm(2) promotes terminal osteogenic differentiation, including rearrangement of F-actin stress fiber, up-regulation of osteogenic genes expression, elevation of alkaline phosphatase activity, secretion of type I collagen and osteoid nodule formation. Moreover, collaboration of BMP2 and integrin β1 pathways plays a significant role in such differentiation processes. Our findings provide further experimental evidence to support the notion that single bout short duration fluid shear stress can promote osteogenic differentiation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0061600