Bacterial population in intestines of the black tiger shrimp (Penaeus monodon) under different growth stages

Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon), bacterial populations of disease-free shrimp were characterized from intestines of four developmenta...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e60802-e60802
Main Authors: Rungrassamee, Wanilada, Klanchui, Amornpan, Chaiyapechara, Sage, Maibunkaew, Sawarot, Tangphatsornruang, Sithichoke, Jiravanichpaisal, Pikul, Karoonuthaisiri, Nitsara
Format: Article
Language:English
Subjects:
Abundance
Agriculture
Analysis
Animals
Aquaculture
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Bacteroides
Bar codes
Bioinformatics
Biology
Biotechnology
Communities
Crustacea
Developmental stages
DNA sequencing
DNA, Bacterial - genetics
Ecosystems
Epidemics
Firmicutes
Gel electrophoresis
Genes
Genetic engineering
Growth
Immune system
Intestine
Intestines
Intestines - microbiology
Juveniles
Laboratories
Larva - growth & development
Larva - microbiology
Larvae
Microbiota
Penaeidae - growth & development
Penaeidae - microbiology
Penaeus monodon
Population
Principal Component Analysis
Probiotics
Proteobacteria
Real time
Relative abundance
Sequence Analysis, DNA
Shellfish
Time Factors
Veterinary Science
Zebrafish
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Summary:Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon), bacterial populations of disease-free shrimp were characterized from intestines of four developmental stages (15-day-old post larvae (PL15), 1- (J1), 2- (J2), and 3-month-old (J3) juveniles) using pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis (DGGE) approaches. A total of 25,121 pyrosequencing reads (reading length = 442±24 bases) were obtained, which were categorized by barcode for PL15 (7,045 sequences), J1 (3,055 sequences), J2 (13,130 sequences) and J3 (1,890 sequences). Bacteria in the phyla Bacteroides, Firmicutes and Proteobacteria were found in intestines at all four growth stages. There were 88, 14, 27, and 20 bacterial genera associated with the intestinal tract of PL15, J1, J2 and J3, respectively. Pyrosequencing analysis revealed that Proteobacteria (class Gammaproteobacteria) was a dominant bacteria group with a relative abundance of 89% for PL15 and 99% for J1, J2 and J3. Real-time PCR assay also confirmed that Gammaproteobacteria had the highest relative abundance in intestines from all growth stages. Intestinal bacterial communities from the three juvenile stages were more similar to each other than that of the PL shrimp based on PCA analyses of pyrosequencing results and their DGGE profiles. This study provides descriptive bacterial communities associated to the black tiger shrimp intestines during these growth development stages in rearing facilities.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0060802