The terpolymer produced by Azotobacter chroococcum 7B: effect of surface properties on cell attachment

The copolymerization of poly(3-hydroxybutyrate) (PHB) is a promising trend in bioengineering to improve biomedical properties, e.g. biocompatibility, of this biodegradable polymer. We used strain Azotobacter chroococcum 7B, an effective producer of PHB, for biosynthesis of not only homopolymer and i...

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Bibliographic Details
Published in:PloS one 2013-02, Vol.8 (2), p.e57200-e57200
Main Authors: Bonartsev, Anton, Yakovlev, Sergey, Boskhomdzhiev, Arasha, Zharkova, Irina, Bagrov, Dmitrii, Myshkina, Vera, Mahina, Tatiana, Kharitonova, Elena, Samsonova, Olga, Zernov, Anton, Zhuikov, Vsevolod, Efremov, Yurii, Voinova, Vera, Bonartseva, Garina, Shaitan, Konstantin
Format: Article
Language:English
Subjects:
Acids
Adsorption
Alcohol
Azotobacter - metabolism
Azotobacter chroococcum
Bacteria
Biochemistry
Biocompatibility
Biocompatible Materials
Biodegradability
Biodegradable materials
Biodegradation
Bioengineering
Biology
Biomedical materials
Biopolymers
Biopolymers - biosynthesis
Biopolymers - physiology
Biosynthesis
Bone surgery
Calorimetry, Differential Scanning
Carbon
Carbon sources
Cell Adhesion
Cell growth
Cell Proliferation
Cell surface
Chemical properties
Chemistry
Contact angle
Copolymerization
Crystallization
Dentistry
Hydrophilicity
Impact strength
Magnetic properties
Magnetic resonance
Magnetic Resonance Spectroscopy
Materials Science
Microscopy, Atomic Force
Molecular Weight
Monomers
NMR
Nuclear magnetic resonance
Physicochemical properties
Polyesters
Polyethylene
Polyethylene glycol
Polyhydroxybutyrate
Polymers
Protein adsorption
Sucrose
Sugar
Surface chemistry
Surface energy
Surface properties
Surface roughness
Terpolymers
Tissue engineering
Valeric acid
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Summary:The copolymerization of poly(3-hydroxybutyrate) (PHB) is a promising trend in bioengineering to improve biomedical properties, e.g. biocompatibility, of this biodegradable polymer. We used strain Azotobacter chroococcum 7B, an effective producer of PHB, for biosynthesis of not only homopolymer and its main copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV), but also novel terpolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-poly(ethylene glycol) (PHB-HV-PEG), using sucrose as the primary carbon source and valeric acid and poly(ethylene glycol) 300 (PEG 300) as additional carbon sources. The chemical structure of PHB-HV-PEG was confirmed by (1)H nuclear-magnetic resonance analysis. The physico-chemical properties (molecular weight, crystallinity, hydrophilicity, surface energy) of produced biopolymer, the protein adsorption to the terpolymer, and cell growth on biopolymer films were studied. Despite of low EG-monomers content in bacterial-origin PHB-HV-PEG polymer, the terpolymer demonstrated significant improvement in biocompatibility in vitro in contrast to PHB and PHB-HV polymers, which may be coupled with increased protein adsorption, hydrophilicity and surface roughness of PEG-containing copolymer.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0057200