Clinicopathological correlation and prognostic significance of protein kinase cα overexpression in human gastric carcinoma

This study investigated the PKCα protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKCα protein expression in gastric carcinoma was analyzed. Quantitative real-time PCR test was applied to compare the PKCα mRNA expression i...

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Bibliographic Details
Published in:PloS one 2013-02, Vol.8 (2), p.e56675-e56675
Main Authors: Lin, Shee-Chan, Chen, Wei-Yu, Lin, Kai-Yuan, Chen, Sheng-Hsuan, Chang, Chun-Chao, Lin, Sey-En, Fang, Chia-Lang
Format: Article
Language:English
Subjects:
Adult
Age Factors
Aged
Apoptosis
Carcinoma - diagnosis
Carcinoma - genetics
Carcinoma - pathology
Cell growth
Correlation analysis
Differentiation
Female
Gastric cancer
Gastroenterology
Gene Expression
Gene Expression Regulation, Neoplastic
Hospitals
Humans
Immunohistochemistry
Immunoreactivity
Internal medicine
Kinases
Male
Medical prognosis
Medicine
Metastases
Metastasis
Middle Aged
Motility
Neoplasm Grading
Neoplasm Staging
Pathology
Patients
Prognosis
Proportional Hazards Models
Protein kinase
Protein kinase C
Protein Kinase C-alpha - genetics
Protein Kinase C-alpha - metabolism
Proteins
Real time
Regression analysis
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stomach cancer
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Survival
Tissues
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Summary:This study investigated the PKCα protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKCα protein expression in gastric carcinoma was analyzed. Quantitative real-time PCR test was applied to compare the PKCα mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKCα protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKCα overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKCα protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKCα protein expression in patients with gastric carcinoma. PKCα mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKCα protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0056675