Rat embryonic mast cells originate in the AGM

Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not k...

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Bibliographic Details
Published in:PloS one 2013-03, Vol.8 (3), p.e57862-e57862
Main Authors: Guiraldelli, Michel Farchi, França, Carolina Nunes, de Souza, Jr, Devandir Antonio, da Silva, Elaine Zayas Marcelino, Toso, Vanina Danuza, Carvalho, Celiane Cardoso, Jamur, Maria Célia, Oliver, Constance
Format: Article
Language:English
Subjects:
Allergies
Animals
Antibodies
Aorta
Aorta - embryology
Aorta - metabolism
Asthma
Biology
Bone marrow
c-Kit protein
CD13 antigen
CD34 antigen
CD40 antigen
Cell adhesion & migration
Cell Differentiation
Coronary vessels
Embryos
Female
Fetuses
Gestation
Gonads - embryology
Gonads - metabolism
Hematopoietic stem cells
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Hypersensitivity
Immunoglobulins
Immunomagnetic Separation
Liver - embryology
Liver - metabolism
Male
Mast cells
Mast Cells - cytology
Mast Cells - metabolism
Medicine
Membrane Glycoproteins - metabolism
Mesonephros - embryology
Mesonephros - metabolism
Molecular biology
Morphology
Osteoprogenitor cells
Pluripotency
Rats
Receptors, Complement - metabolism
Receptors, IgE - genetics
Receptors, IgE - metabolism
Rodents
Stem cell transplantation
Stem cells
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Summary:Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not known. In the present study, we identified MCcps in rat embryos using these previously characterized mast cell specific antibodies. The MCcps were found in the AGM (aorta-gonad-mesonephros) region of rat embryos at E11.5. These cells were BGD6+, CD34(+), c-kit(+), CD13(+), FcεRI(-), AA4(-) CD40(-), and Thy-1(-). By PCR the cells contained message for the α and β subunits of FcεRI and mast cell specific proteases. In vitro, the MCcps differentiated into metachromatic mast cells. With age of gestation the percent of MCcps diminished while the percent of mast cell progenitors increased. An increased knowledge of the biology and embryonic origin of mast cells may contribute to a greater understanding of allergy, asthma, and other mast cell related diseases.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0057862