Resident CD11b(+)Ly6C(-) lung dendritic cells are responsible for allergic airway sensitization to house dust mite in mice

Conventional dendritic cells (DCs) are considered to be the prime initiators of airway allergy. Yet, it remains unclear whether specific DC subsets are preferentially involved in allergic airway sensitization. Here, we systematically assessed the respective pro-allergic potential of individually sor...

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Bibliographic Details
Published in:	PloS one 2012, Vol.7 (12), p.e53242-e53242
Main Authors:	Mesnil, Claire, Sabatel, Catherine M, Marichal, Thomas, Toussaint, Marie, Cataldo, Didier, Drion, Pierre-Vincent, Lekeux, Pierre, Bureau, Fabrice, Desmet, Christophe J
Format:	Article
Language:	eng
Subjects:	Allergies Animals Antigens Antigens, Dermatophagoides - immunology Antigens, Dermatophagoides - metabolism Asthma Biochemistry, biophysics & molecular biology Biochimie, biophysique & biologie moléculaire Biology Bone marrow CD103 antigen CD11b antigen CD11b Antigen - metabolism CD11c antigen Chemokines Cytokines Dendritic cells Dendritic Cells - immunology Dendritic Cells - metabolism Dust Female Homeostasis House dust Hypersensitivity Hypersensitivity - immunology Hypersensitivity - metabolism Immunology Inflammation Initiators Laboratories Life sciences Ligands Lung - immunology Lung - metabolism Lungs Lymph nodes Lymphatic system Lymphocytes Lymphocytes T Mice Mice, Inbred C57BL Mites Monocytes Muridae Pyroglyphidae - immunology Pyroglyphidae - metabolism Respiratory System - immunology Respiratory System - metabolism Respiratory tract Rodents Sciences du vivant T cell receptors Veterinary medicine
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Summary:	Conventional dendritic cells (DCs) are considered to be the prime initiators of airway allergy. Yet, it remains unclear whether specific DC subsets are preferentially involved in allergic airway sensitization. Here, we systematically assessed the respective pro-allergic potential of individually sorted lung DC subsets isolated from house dust mite antigen (HDM)-treated donor mice, following transfer to naïve recipients. Transfer of lung CD11c(+)CD11b(+) DCs, but not CD11c(+)CD11b(-)CD103(+) DCs, was sufficient to prime airway allergy. The CD11c(+)CD11b(+) DC subpopulation was composed of CD11c(+)CD11b(+)Ly6C(+) inflammatory monocyte-derived cells, whose numbers increase in the lungs following HDM exposure, and of CD11c(+)CD11b(+)Ly6C(-) DCs, which remain stable. Counterintuitively, only CD11c(+)CD11b(+)Ly6C(-) DCs, and not CD11c(+)CD11b(+)Ly6C(+) DCs, were able to convey antigen to the lymph nodes and induce adaptive T cell responses and subsequent airway allergy. Our results thus support that lung resident non-inflammatory CD11c(+)CD11b(+)Ly6C(-) DCs are the essential inducers of allergic airway sensitization to the common aeroallergen HDM in mice.
ISSN:	1932-6203 1932-6203