Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay....

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Bibliographic Details
Published in:PloS one 2012-12, Vol.7 (12), p.e50799-e50799
Main Authors: Palacín, Arantxa, Gómez-Casado, Cristina, Rivas, Luis A, Aguirre, Jacobo, Tordesillas, Leticia, Bartra, Joan, Blanco, Carlos, Carrillo, Teresa, Cuesta-Herranz, Javier, de Frutos, Consolación, Alvarez-Eire, Genoveva García, Fernández, Francisco J, Gamboa, Pedro, Muñoz, Rosa, Sánchez-Monge, Rosa, Sirvent, Sofía, Torres, María J, Varela-Losada, Susana, Rodríguez, Rosalía, Parro, Victor, Blanca, Miguel, Salcedo, Gabriel, Díaz-Perales, Araceli
Format: Article
Language:English
Subjects:
Allergens
Allergens - chemistry
Allergens - immunology
Allergies
Allergy
Al·lèrgens
Analysis
Antigens, Plant - immunology
Care and treatment
Carrier Proteins - immunology
Cloning
Computer Science
Cross Reactions - immunology
Cross-reactivity
Electrophoresis, Polyacrylamide Gel
Epitopes - chemistry
Fertilizer industry
Fertilizers
Food
Food allergies
Food hypersensitivity
Food Hypersensitivity - immunology
Food plants
Fruits
Geography
Humans
Immunoassay
Immunoassay - methods
Immunologia
Immunology
Laboratories
Lipid transfer proteins
Lipids
Lipids - chemistry
Medical research
Medicine
Models, Statistical
Patients
Plant proteins
Plant Proteins - immunology
Pollen
Protein Array Analysis - methods
Protein arrays
Proteins
Proteïnes vegetals
Reactivity
Recombinant Proteins - chemistry
Spain
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0050799