Aging and estrogen status: a possible endothelium-dependent vascular coupling mechanism in bone remodeling

Bone loss with aging and menopause may be linked to vascular endothelial dysfunction. The purpose of the study was to determine whether putative modifications in endothelium-dependent vasodilation of the principal nutrient artery (PNA) of the femur are associated with changes in trabecular bone volu...

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Published in:PloS one 2012-11, Vol.7 (11), p.e48564-e48564
Main Authors: Prisby, Rhonda D, Dominguez, 2nd, James M, Muller-Delp, Judy, Allen, Matthew R, Delp, Michael D
Format: Article
Language:English
Subjects:
Acetylcholine
Age
Aging
Aging - blood
Aging - metabolism
Animals
Arteries - drug effects
Arteries - physiology
Biology
Bone (trabecular)
Bone and Bones - drug effects
Bone and Bones - physiology
Bone loss
Bone remodeling
Bone Remodeling - drug effects
Bone Remodeling - physiology
Cancellous bone
Computed tomography
Coupling
Cyclooxygenase Inhibitors - pharmacology
Endothelium
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Enzyme Inhibitors - pharmacology
Estrogen Replacement Therapy
Estrogens
Estrogens - blood
Estrogens - metabolism
Female
Females
Femur
Hormone replacement therapy
Hydrazines - pharmacology
Kinesiology
Laboratory animals
Medicine
Menopause
Metaphysis
NG-Nitroarginine Methyl Ester - pharmacology
Nitric oxide
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase - metabolism
Organ Size - drug effects
Osteoporosis
Ovariectomy
Phenols (Class of compounds)
Physiology
Pulmonary arteries
Rats
Rats, Inbred F344
Regression Analysis
Rodents
Vasodilation
Vasodilator Agents - pharmacology
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Summary:Bone loss with aging and menopause may be linked to vascular endothelial dysfunction. The purpose of the study was to determine whether putative modifications in endothelium-dependent vasodilation of the principal nutrient artery (PNA) of the femur are associated with changes in trabecular bone volume (BV/TV) with altered estrogen status in young (6 mon) and old (24 mon) female Fischer-344 rats. Animals were divided into 6 groups: 1) young intact, 2) old intact, 3) young ovariectomized (OVX), 4) old OVX, 5) young OVX plus estrogen replacement (OVX+E2), and 6) old OVX+E2. PNA endothelium-dependent vasodilation was assessed in vitro using acetylcholine. Trabecular bone volume of the distal femoral metaphysis was determined by microCT. In young rats, vasodilation was diminished by OVX and restored with estrogen replacement (intact, 82±7; OVX, 61±9; OVX+E2, 90±4%), which corresponded with similar modifications in BV/TV (intact, 28.7±1.6; OVX, 16.3±0.9; OVX+E2, 25.7±1.4%). In old animals, vasodilation was unaffected by OVX but enhanced with estrogen replacement (intact, 55±8; OVX, 59±7; OVX+E2, 92±4%). Likewise, modifications in BV/TV followed the same pattern (intact, 33.1±1.6; OVX, 34.4±3.7; OVX+E2, 42.4±2.1%). Furthermore, in old animals with low endogenous estrogen (i.e., intact and old OVX), vasodilation was correlated with BV/TV (R(2) = 0.630; P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0048564