A characterization of the oral microbiome in allogeneic stem cell transplant patients

The mouth is a complex biological structure inhabited by diverse bacterial communities. The purpose of this study is to describe the effects of allogeneic stem cell transplantation on the oral microbiota and to examine differences among those patients who acquired respiratory complications after tra...

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Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e47628-e47628
Main Authors: Ames, Nancy J, Sulima, Pawel, Ngo, Thoi, Barb, Jennifer, Munson, Peter J, Paster, Bruce J, Hart, Thomas C
Format: Article
Language:English
Subjects:
Adult
Analysis
Bacteria
Bacteria - classification
Bacteria - genetics
Biology
Cancer
Cluster Analysis
Complications
Deoxyribonucleic acid
Disease
DNA
DNA microarrays
Female
Gene expression
Gene Expression Profiling
Humans
Immune system
Information technology
Intensive care
Laboratories
Male
Medicine
Microbiota
Microbiota (Symbiotic organisms)
Microbiota - genetics
Microorganisms
Middle Aged
Mortality
Mouth - microbiology
Nosocomial infections
Oligonucleotide Array Sequence Analysis
Pathogens
Patients
Principal Component Analysis
Principal components analysis
Respiratory System - microbiology
RNA, Ribosomal, 16S - genetics
Signs and symptoms
Statistical analysis
Stem cell transplantation
Stem Cell Transplantation - methods
Stem cells
Studies
Taxa
Teeth
Time Factors
Transplantation
Transplantation, Homologous
Transplants & implants
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Summary:The mouth is a complex biological structure inhabited by diverse bacterial communities. The purpose of this study is to describe the effects of allogeneic stem cell transplantation on the oral microbiota and to examine differences among those patients who acquired respiratory complications after transplantation. All patients were consented at the National Institutes of Health, Clinical Center. Bacterial DNA was analyzed from patients' oral specimens using the Human Oral Microbe Identification Microarray. The specimens were collected from four oral sites in 45 allogeneic transplantation patients. Specimens were collected at baseline prior to transplantation, after transplantation at the nadir of the neutrophil count and after myeloid engraftment. If respiratory signs and symptoms developed, additional specimens were obtained. Patients were followed for 100 days post transplantation. Eleven patients' specimens were subjected to further statistical analysis. Many common bacterial genera, such as Streptococcus, Veillonella, Gemella, Granulicatella and Camplyobacter were identified as being present before and after transplantation. Five of 11 patients developed respiratory complications following transplantation and there was preliminary evidence that the oral microbiome changed in their oral specimens. Cluster analysis and principal component analysis revealed this change in the oral microbiota. After allogeneic transplantation, the oral bacterial community's response to a new immune system was not apparent and many of the most common core oral taxa remained unaffected. However, the oral microbiome was affected in patients who developed respiratory signs and symptoms after transplantation. The association related to the change in the oral microbiota and respiratory complications after transplantation will be validated by future studies using high throughput molecular methods.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0047628