Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei

Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 c...

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Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e47038-e47038
Main Authors: Zhang, Shuang, Li, Chao-Zheng, Yan, Hui, Qiu, Wei, Chen, Yong-Gui, Wang, Pei-Hui, Weng, Shao-Ping, He, Jian-Guo
Format: Article
Language:English
Subjects:
Activation
Agriculture
Amino Acid Sequence
Animals
Antiinfectives and antibacterials
Antimicrobial Cationic Peptides - genetics
Base Sequence
Biology
Blood cells
Cloning
Cloning, Molecular
CpG islands
Crustaceans
Cytokines
Deoxyribonucleic acid
Differentiation
DNA
Drosophila
Drosophila - cytology
Drosophila - genetics
Gene expression
Gene Expression Regulation
Genes
Hemocytes
Hemocytes - drug effects
Hemocytes - physiology
Hemocytes - virology
Hepatopancreas
Identification and classification
IE1 protein
Immune system
Infections
Interleukin
Interleukin 1
Invertebrates
Laboratories
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Litopenaeus vannamei
Low level
Molecular Sequence Data
MyD88 protein
Myeloid Differentiation Factor 88 - genetics
Myeloid Differentiation Factor 88 - metabolism
NF-κB protein
Penaeidae - genetics
Penaeidae - immunology
Penaeidae - metabolism
Penaeidae - microbiology
Penaeidae - virology
Penaeus monodon
Peptides
Phylogeny
Physiological aspects
Promoter Regions, Genetic
Properties
Proteins
Sequence Homology, Amino Acid
Shellfish
Signal Transduction
Signaling
Signaling peptides and proteins
Staphylococcus aureus
Staphylococcus aureus - pathogenicity
Structure
Toll-like receptors
Transcription activation
Vibrio parahaemolyticus
Vibrio parahaemolyticus - pathogenicity
Viruses
Water-borne diseases
Waterborne diseases
White spot syndrome
White spot syndrome virus
White spot syndrome virus 1 - pathogenicity
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Summary:Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp in length and contains an open reading frame (ORF) of 1,428 bp, whereas the LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants encode proteins with death and Toll/interleukin-1 receptor domains and share 91% sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s expression was induced in hemocytes after challenge with lipopolysaccharide, CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot syndrome virus, but not by poly I∶C. Overexpression of LvMyD88 and LvMyD88-1 in Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a role in antibacterial and antiviral response in L. vannamei. To our knowledge, this is the first report on MyD88 in shrimp and a variant of MyD88 gene in invertebrates.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0047038