Transition pattern and mechanism of B-lymphocyte precursors in regenerated mouse bone marrow after subtotal body irradiation

Little is known about the effects of ionizing radiation on the transition and the related signal transduction of progenitor B cells in the bone marrow. Thus, using an NIH Swiss mouse model, we explored the impact of ionizing radiation on the early stage of B-cell development via an examination of th...

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Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e46560-e46560
Main Authors: Han, Deping, Zhang, Mei, Ma, Jun, Hong, Jingshen, Chen, Chun, Zhang, Bingrong, Huang, Luqiang, Lv, Wenlong, Yin, Liangjie, Zhang, Amy, Zhang, Hengshan, Zhang, Zhenhuan, Vidyasagar, Sadasivan, Okunieff, Paul, Zhang, Lurong
Format: Article
Language:English
Subjects:
Analysis
Animals
B cells
Biology
Bone marrow
Bone Marrow - physiology
Bone Marrow - radiation effects
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Bone Marrow Cells - radiation effects
Cancer
Cell Count
Cell cycle
Cell differentiation
Chains
Cytokines
Developmental stages
Gene Rearrangement, B-Lymphocyte
Immunoglobulins
Immunology
Ionizing radiation
Irradiation
Kinases
Light
Lymphocytes
Lymphocytes B
Lymphoid cells
Male
Mice
Oncology
Osteoprogenitor cells
Precursor Cells, B-Lymphoid - cytology
Precursor Cells, B-Lymphoid - metabolism
Precursor Cells, B-Lymphoid - radiation effects
Precursors
Radiation
Radiation dosage
Receptors, Interleukin-7 - metabolism
Regeneration - radiation effects
Signal transduction
Signal Transduction - radiation effects
Signaling
Stem cells
Transcription factors
Transcription Factors - metabolism
Transduction
Whole-Body Irradiation
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Summary:Little is known about the effects of ionizing radiation on the transition and the related signal transduction of progenitor B cells in the bone marrow. Thus, using an NIH Swiss mouse model, we explored the impact of ionizing radiation on the early stage of B-cell development via an examination of the transition of CLP to pro-B to pre-B cells within bone marrow as a function of radiation doses and times. Our results showed that while the total number of bone marrow lymphoid cells at different stages were greatly reduced by subtotal body irradiation (sub-TBI), the surviving cells continued to transition from common lymphoid progenitors to pro-B and then to pre-B in a reproducible temporal pattern. The rearrangement of the immunoglobulin heavy chain increased significantly 1-2 weeks after irradiation, but no change occurred after 3-4 weeks. The rearrangement of the immunoglobulin light chain decreased significantly 1-2 weeks after sub-TBI but increased dramatically after 3-4 weeks. In addition, several key transcription factors and signaling pathways were involved in B-precursor transitions after sub-TBI. The data indicate that week 2 after irradiation is a critical time for the transition from pro-B cells to pre-B cells, reflecting that the functional processes for different B-cell stages are well preserved even after high-dose irradiation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0046560