Recruitment of Rpd3 to the telomere depends on the protein arginine methyltransferase Hmt1

In the yeast Saccharomyces cerevisiae, the establishment and maintenance of silent chromatin at the telomere requires a delicate balance between opposing activities of histone modifying enzymes. Previously, we demonstrated that the protein arginine methyltransferase Hmt1 plays a role in the formatio...

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Bibliographic Details
Published in:PloS one 2012-08, Vol.7 (8), p.e44656-e44656
Main Authors: Milliman, Eric J, Yadav, Neelu, Chen, Yin-Chu, Muddukrishna, Bhavana, Karunanithi, Sheelarani, Yu, Michael C
Format: Article
Language:English
Subjects:
Acetylation
Arginine
Baking yeast
Biology
Catalysis
Catalytic activity
Chromatin
Chromatin Immunoprecipitation
Epigenetics
Epistasis
Epistasis, Genetic
Gene expression
Gene Silencing
Genes, Synthetic - genetics
Genetic aspects
Genetic screening
Genetic Testing
Genome, Fungal - genetics
Genomes
Histone deacetylase
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Histones - metabolism
Kinases
Methylation
Methyltransferases
Mutants
Mutation - genetics
Ontology
Physiological aspects
Protein arginine methyltransferase
Protein Binding
Protein Subunits - metabolism
Protein-Arginine N-Methyltransferases - genetics
Protein-Arginine N-Methyltransferases - metabolism
Proteins
Repressor Proteins - genetics
Repressor Proteins - metabolism
Ribosomal DNA
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism
Sirtuin 2 - metabolism
Substrates
Telomere - metabolism
Telomeres
Yeast
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Summary:In the yeast Saccharomyces cerevisiae, the establishment and maintenance of silent chromatin at the telomere requires a delicate balance between opposing activities of histone modifying enzymes. Previously, we demonstrated that the protein arginine methyltransferase Hmt1 plays a role in the formation of yeast silent chromatin. To better understand the nature of the Hmt1 interactions that contribute to this phenomenon, we carried out a systematic reverse genetic screen using a null allele of HMT1 and the synthetic genetic array (SGA) methodology. This screen revealed interactions between HMT1 and genes encoding components of the histone deacetylase complex Rpd3L (large). A double mutant carrying both RPD3 and HMT1 deletions display increased telomeric silencing and Sir2 occupancy at the telomeric boundary regions, when comparing to a single mutant carrying Hmt1-deletion only. However, the dual rpd3/hmt1-null mutant behaves like the rpd3-null single mutant with respect to silencing behavior, indicating that RPD3 is epistatic to HMT1. Mutants lacking either Hmt1 or its catalytic activity display an increase in the recruitment of histone deacetylase Rpd3 to the telomeric boundary regions. Moreover, in such loss-of-function mutants the levels of acetylated H4K5, which is a substrate of Rpd3, are altered at the telomeric boundary regions. In contrast, the level of acetylated H4K16, a target of the histone deacetylase Sir2, was increased in these regions. Interestingly, mutants lacking either Rpd3 or Sir2 display various levels of reduction in dimethylated H4R3 at these telomeric boundary regions. Together, these data provide insight into the mechanism whereby Hmt1 promotes the proper establishment and maintenance of silent chromatin at the telomeres.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0044656