Expression patterns of Anaplasma marginale Msp2 variants change in response to growth in cattle, and tick cells versus mammalian cells

Antigenic variation of major surface proteins is considered an immune-evasive maneuver used by pathogens as divergent as bacteria and protozoa. Likewise, major surface protein 2 (Msp2) of the tick-borne pathogen, Anaplasma marginale, is thought to be involved in antigenic variation to evade the mamm...

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Bibliographic Details
Published in:PloS one 2012-04, Vol.7 (4), p.e36012-e36012
Main Authors: Chávez, Adela S Oliva, Felsheim, Roderick F, Kurtti, Timothy J, Ku, Pei-Shin, Brayton, Kelly A, Munderloh, Ulrike G
Format: Article
Language:English
Subjects:
Alleles
Allen, Paul G
Amino Acid Sequence
Amino acids
Analysis
Anaplasma
Anaplasma marginale - growth & development
Anaplasma marginale - immunology
Anaplasma phagocytophilum
Anaplasmosis - blood
Anaplasmosis - microbiology
Animals
Antibodies, Bacterial - immunology
Antigenic Variation - immunology
Antigens
Antigens, Bacterial - chemistry
Antigens, Bacterial - metabolism
Arachnids
Bacteria
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - metabolism
Biology
Blood
Borrelia burgdorferi
Bovidae
Cattle
Cell Line
Cell lines
Cells (Biology)
Colonies
Colony Count, Microbial
Computational Biology
Gene expression
Genes
Genomes
Genomics
Host systems
Immune response
Immune system
Immunofluorescence
Infection
Infections
Lyme disease
Mammalian cells
Mammals
Mammals - microbiology
Medicine
Molecular Sequence Data
Molecular weight
Monoclonal antibodies
Pathogens
Protein structure
Protein Structure, Secondary
Proteins
Protozoa
Rickettsia
Secondary structure
Sequence Alignment
Species Specificity
Ticks
Ticks - microbiology
Veterinary Science
Western blotting
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Summary:Antigenic variation of major surface proteins is considered an immune-evasive maneuver used by pathogens as divergent as bacteria and protozoa. Likewise, major surface protein 2 (Msp2) of the tick-borne pathogen, Anaplasma marginale, is thought to be involved in antigenic variation to evade the mammalian host immune response. However, this dynamic process also works in the tick vector in the absence of immune selection pressure. We examined Msp2 variants expressed during infection of four tick and two mammalian cell-lines to determine if the presence of certain variants correlated with specific host cell types. Anaplasma marginale colonies differed in their development and appearance in each of the cell lines (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0036012