The Cryptosporidium parvum transcriptome during in vitro development

Cryptosporidiosis is caused by an obligate intracellular parasite that has eluded global transcriptional or proteomic analysis of the intracellular developmental stages. The transcript abundance for 3,302 genes (87%) of the Cryptosporidium parvum protein coding genome was elucidated over a 72 hr inf...

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Bibliographic Details
Published in:PloS one 2012-03, Vol.7 (3), p.e31715-e31715
Main Authors: Mauzy, Mary J, Enomoto, Shinichiro, Lancto, Cheryl A, Abrahamsen, Mitchell S, Rutherford, Mark S
Format: Article
Language:English
Subjects:
Analysis
Base Sequence
Bioinformatics
Biology
Cell Line
Cluster Analysis
Clustering
Clusters
Cryptosporidiosis
Cryptosporidiosis - parasitology
Cryptosporidium
Cryptosporidium parvum
Cryptosporidium parvum - genetics
Cryptosporidium parvum - growth & development
Cryptosporidium parvum - pathogenicity
Deoxyribonucleic acid
Developmental stages
DNA
Epithelial cells
Epithelial Cells - parasitology
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes
Genome, Protozoan
Genomes
Genomics
Health aspects
Host-Parasite Interactions - genetics
Humans
Identification methods
Infection
Infections
Intestine
Intestines - parasitology
Intracellular
Medicine
Merozoites - growth & development
Parasites
Plasmodium falciparum
Proteasomes
Proteins
Proteomics
Real-Time Polymerase Chain Reaction
RNA
RNA, Protozoan - genetics
RNA, Ribosomal, 18S - genetics
rRNA 18S
Sporozoites - growth & development
Standardization
Structural proteins
Theileria
Toxoplasma gondii
Transcription
Transcription (Genetics)
Transcription factors
Transcriptome
Trophozoites - growth & development
Veterinary medicine
Veterinary Science
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Summary:Cryptosporidiosis is caused by an obligate intracellular parasite that has eluded global transcriptional or proteomic analysis of the intracellular developmental stages. The transcript abundance for 3,302 genes (87%) of the Cryptosporidium parvum protein coding genome was elucidated over a 72 hr infection within HCT8 cells using Real Time-PCR. The parasite had detectable transcription of all genes in vitro within at least one time point tested, and adjacent genes were not co-regulated. Five genes were not detected within the first 24 hr of infection, one containing two AP2 domains. The fewest genes detected were at 2 hr post infection, while 30% (985) of the genes have their highest expression at 48 and/or 72 hr. Nine expression clusters were formed over the entire 72 hr time course and indicate patterns of transcriptional increases at each of the 7 time points collected except 36 hr, including genes paralleling parasite 18S rRNA transcript levels. Clustering within only the first 24 hr of infection indicates spikes in expression at each of the 4 time points, a group paralleling 18S rRNA transcript levels, and a cluster with peaks at both 6 and 24 hr. All genes were classified into 18 functional categories, which were unequally distributed across clusters. Expression of metabolic, ribosomal and proteasome proteins did not parallel 18S rRNA levels indicating distinct biochemical profiles during developmental stage progression. Proteins involved in translation are over-represented at 6 hr, while structural proteins are over-represented at 12 hr. Standardization methods identified 107 genes with
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0031715