Computational and serologic analysis of novel and known viruses in species human adenovirus D in which serology and genomics do not correlate

In November of 2007 a human adenovirus (HAdV) was isolated from a bronchoalveolar lavage (BAL) sample recovered from a biopsy of an AIDS patient who presented with fever, cough, tachycardia, and expiratory wheezes. To better understand the isolated virus, the genome was sequenced and analyzed using...

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Bibliographic Details
Published in:	PloS one 2012-03, Vol.7 (3), p.e33212-e33212
Main Authors:	Liu, Elizabeth B, Wadford, Debra A, Seto, Jason, Vu, Maria, Hudson, Nolan Ryan, Thrasher, Lisa, Torres, Sarah, Dyer, David W, Chodosh, James, Seto, Donald, Jones, Morris S
Format:	Article
Language:	eng
Subjects:	Acquired immune deficiency syndrome Adenoviruses Adenoviruses, Human - classification Adenoviruses, Human - genetics AIDS AIDS Serodiagnosis - methods AIDS-Related Opportunistic Infections - virology Alveoli Analysis Archives & records Base Sequence Bioinformatics Biology Biopsy Bronchoalveolar Lavage Fluid - virology Bronchus Capsid Proteins - genetics Computational Biology Computer applications Conformation Cough Disease Fever Genes Genome, Viral - genetics Genomes Genomics Genomics - methods Human adenovirus Humans Hyperthermia Laboratories Medicine Molecular Sequence Data Neutralization Neutralization Tests Proteins Public health Recombination Sequence Analysis, DNA Sequence Homology Serology Species Specificity Tachycardia Taxonomy Viruses
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Summary:	In November of 2007 a human adenovirus (HAdV) was isolated from a bronchoalveolar lavage (BAL) sample recovered from a biopsy of an AIDS patient who presented with fever, cough, tachycardia, and expiratory wheezes. To better understand the isolated virus, the genome was sequenced and analyzed using bioinformatic and phylogenomic analysis. The results suggest that this novel virus, which is provisionally named HAdV-D59, may have been created from multiple recombination events. Specifically, the penton, hexon, and fiber genes have high nucleotide identity to HAdV-D19C, HAdV-D25, and HAdV-D56, respectively. Serological results demonstrated that HAdV-D59 has a neutralization profile that is similar yet not identical to that of HAdV-D25. Furthermore, we observed a two-fold difference between the ability of HAdV-D15 and HAdV-D25 to be neutralized by reciprocal antiserum indicating that the two hexon proteins may be more similar in epitopic conformation than previously assumed. In contrast, hexon loops 1 and 2 of HAdV-D15 and HAdV-D25 share 79.13 and 92.56 percent nucleotide identity, respectively. These data suggest that serology and genomics do not always correlate.
ISSN:	1932-6203 1932-6203