Somatic ‘Soluble’ Adenylyl Cyclase Isoforms Are Unaffected in Sacytm1Lex/Sacytm1Lex ‘Knockout’ Mice

Background Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacytm1Lex/Sacytm1Lex knockout mi...

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Bibliographic Details
Published in:PloS one 2008-09, Vol.3 (9), p.e3251
Main Authors: Farrell, Jeanne, Ramos, Lavoisier, Tresguerres, Martin, Kamenetsky, Margarita, Levin, Lonny R., Buck, Jochen
Format: Article
Language:English
Subjects:
Animal tissues
Biochemistry/Cell Signaling and Trafficking Structures
Cell Biology/Cell Signaling
Clonal deletion
Crystal structure
Exons
Gene expression
Genetics and Genomics/Gene Discovery
Genomes
Germ cells
Immunology
Isoforms
Mammals
Molecular Biology
Monoclonal antibodies
Nervous system
Neuroscience/Neuronal Signaling Mechanisms
Pharmacology
Physiology
Proteins
Ribonucleic acid
RNA
RNA-mediated interference
Rodents
Sperm
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Summary:Background Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from the widely studied, G protein-regulated transmembrane adenylyl cyclases. Genetic deletion of the second through fourth coding exons in Sacytm1Lex/Sacytm1Lex knockout mice results in a male sterile phenotype. The absence of any major somatic phenotype is inconsistent with the variety of somatic functions identified for sAC using pharmacological inhibitors and RNA interference. Principal Findings We now use immunological and molecular biological methods to demonstrate that somatic tissues express a previously unknown isoform of sAC, which utilizes a unique start site, and which ‘escapes’ the design of the Sacytm1Lex knockout allele. Conclusions/Significance These studies reveal increased complexity at the sAC locus, and they suggest that the known isoforms of sAC play a unique function in male germ cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0003251