MiR-29a inhibits cell proliferation and induces cell cycle arrest through the downregulation of p42.3 in human gastric cancer

As a newly identified and characterized gene, p42.3 is associated with cell proliferation and tumorigenicity. The expression of p42.3 is upregulated in human gastric cancer (GC), but its underlying mechanisms of action are not well understood. MicroRNAs (miRNAs) are known to play vital regulatory ro...

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Bibliographic Details
Published in:PloS one 2011-10, Vol.6 (10), p.e25872-e25872
Main Authors: Cui, Yun, Su, Wen-Yu, Xing, Jing, Wang, Ying-Chao, Wang, Ping, Chen, Xiao-Yu, Shen, Zhi-Yong, Cao, Hui, Lu, You-Yong, Fang, Jing-Yuan
Format: Article
Language:English
Subjects:
3' Untranslated regions
3' Untranslated Regions - genetics
Aged
Arsenic
Base Sequence
Bioinformatics
Biology
Brain cancer
Brain research
Cancer
Cell cycle
Cell Cycle Checkpoints - genetics
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell division
Cell growth
Cell Line, Tumor
Cell Proliferation
Computational Biology
Diabetes
Disease
Down-Regulation - genetics
Female
Gastric cancer
Gastroenterology
Gene expression
Gene Expression Regulation, Neoplastic - genetics
Genes
Genetic aspects
Genomes
Genomics
Hepatology
Hospitals
Humans
Leukemia
Male
Medical research
Medicine
Methods
MicroRNA
MicroRNAs - genetics
Middle Aged
miRNA
mRNA
Protein binding
Proteins
RNA polymerase
Stem cells
Stomach cancer
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Studies
Tumor cell lines
Tumorigenicity
Tumors
Citations: Items that cite this one
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Summary:As a newly identified and characterized gene, p42.3 is associated with cell proliferation and tumorigenicity. The expression of p42.3 is upregulated in human gastric cancer (GC), but its underlying mechanisms of action are not well understood. MicroRNAs (miRNAs) are known to play vital regulatory roles in many cellular processes. Here we utilized bioinformatics and experimental approaches to investigate the regulatory relationship between miRNAs and the p42.3 gene. We showed that miR-29a could repress p42.3 expression at both the mRNA and protein levels via directly binding to its 3'UTR. Furthermore, an inverse relationship was observed between miR-29a and p42.3 expression in gastric cancer cell lines and GC tissue samples, especially in cases where p42.3 was downregulated. Taken together, we have elucidated previously unrecognized roles of miR-29a and indicated that miR-29a may function, at least partially, by targeting the p42.3 gene in human GC.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0025872