Drosophila Ten-m and filamin affect motor neuron growth cone guidance

The Drosophila Ten-m (also called Tenascin-major, or odd Oz (odz)) gene has been associated with a pair-rule phenotype. We identified and characterized new alleles of Drosophila Ten-m to establish that this gene is not responsible for segmentation defects but rather causes defects in motor neuron ax...

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Bibliographic Details
Published in:PloS one 2011-08, Vol.6 (8), p.e22956-e22956
Main Authors: Zheng, Lihua, Michelson, Yehudit, Freger, Vita, Avraham, Ziva, Venken, Koen J T, Bellen, Hugo J, Justice, Monica J, Wides, Ron
Format: Article
Language:English
Subjects:
Actin
Animals
Animals, Genetically Modified
Axon guidance
Biology
Body Patterning - genetics
Central nervous system
Cones
Contractile Proteins - genetics
Contractile Proteins - metabolism
Defects
Drosophila
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Ectoderm - cytology
Ectoderm - embryology
Ectoderm - metabolism
Embryo, Nonmammalian - embryology
Embryo, Nonmammalian - metabolism
Embryogenesis
Embryonic development
Embryonic growth stage
Female
Filamins
Fluorescent Antibody Technique
Genes
Genetic aspects
Genetics
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Growth
Growth cones
Growth Cones - metabolism
Immunohistochemistry
Insects
Life sciences
M protein
Male
Medicine
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
Motor Neurons - metabolism
Mutants
Mutation
Neurons
Overexpression
Phenotype
Phenotypes
Protein Binding
Proteins
Rodents
Routing
Segmentation
Tenascin
Tenascin - genetics
Tenascin - metabolism
Transcription factors
Trends
Two-Hybrid System Techniques
Vertebrates
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Summary:The Drosophila Ten-m (also called Tenascin-major, or odd Oz (odz)) gene has been associated with a pair-rule phenotype. We identified and characterized new alleles of Drosophila Ten-m to establish that this gene is not responsible for segmentation defects but rather causes defects in motor neuron axon routing. In Ten-m mutants the inter-segmental nerve (ISN) often crosses segment boundaries and fasciculates with the ISN in the adjacent segment. Ten-m is expressed in the central nervous system and epidermal stripes during the stages when the growth cones of the neurons that form the ISN navigate to their targets. Over-expression of Ten-m in epidermal cells also leads to ISN misrouting. We also found that Filamin, an actin binding protein, physically interacts with the Ten-m protein. Mutations in cheerio, which encodes Filamin, cause defects in motor neuron axon routing like those of Ten-m. During embryonic development, the expression of Filamin and Ten-m partially overlap in ectodermal cells. These results suggest that Ten-m and Filamin in epidermal cells might together influence growth cone progression.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0022956