Mammalian stem cells reprogramming in response to terahertz radiation

We report that extended exposure to broad-spectrum terahertz radiation results in specific changes in cellular functions that are closely related to DNA-directed gene transcription. Our gene chip survey of gene expression shows that whereas 89% of the protein coding genes in mouse stem cells do not...

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Bibliographic Details
Published in:PloS one 2010-12, Vol.5 (12), p.e15806-e15806
Main Authors: Bock, Jonathan, Fukuyo, Yayoi, Kang, Sona, Phipps, M Lisa, Alexandrov, Ludmil B, Rasmussen, Kim Ø, Bishop, Alan R, Rosen, Evan D, Martinez, Jennifer S, Chen, Hou-Tong, Rodriguez, George, Alexandrov, Boian S, Usheva, Anny
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
BASIC BIOLOGICAL SCIENCES
Bioinformatics
Biology
Breathing
Cell cycle
Cell Differentiation
Chemistry
Cloning
Computer Simulation
Cytoplasm - metabolism
Deoxyribonucleic acid
Differentiation (biology)
DNA
DNA - genetics
DNA microarrays
DNA probes
Electromagnetic radiation
Endocrinology
Gene expression
Gene Expression Regulation
Genes
Genetic aspects
Genomics
Influence
Irradiation
Laboratories
Mathematical models
Medical schools
Medicine
Mesenchymal stem cells
Mesoderm - metabolism
Mice
Molecular dynamics
Oligonucleotide Array Sequence Analysis
Peroxisome proliferator-activated receptors
Phenotypes
Physics
Polymerase chain reaction
PPAR gamma - metabolism
Proteins
Radiation
Reverse Transcriptase Polymerase Chain Reaction
Science & Technology - Other Topics
Stem cell transplantation
Stem cells
Stem Cells - cytology
Stem Cells - radiation effects
Terahertz Radiation
Transcription (Genetics)
Transcription factors
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Summary:We report that extended exposure to broad-spectrum terahertz radiation results in specific changes in cellular functions that are closely related to DNA-directed gene transcription. Our gene chip survey of gene expression shows that whereas 89% of the protein coding genes in mouse stem cells do not respond to the applied terahertz radiation, certain genes are activated, while other are repressed. RT-PCR experiments with selected gene probes corresponding to transcripts in the three groups of genes detail the gene specific effect. The response was not only gene specific but also irradiation conditions dependent. Our findings suggest that the applied terahertz irradiation accelerates cell differentiation toward adipose phenotype by activating the transcription factor peroxisome proliferator-activated receptor gamma (PPARG). Finally, our molecular dynamics computer simulations indicate that the local breathing dynamics of the PPARG promoter DNA coincides with the gene specific response to the THz radiation. We propose that THz radiation is a potential tool for cellular reprogramming.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0015806