Hydrogen sulfide attenuated tumor necrosis factor-α-induced inflammatory signaling and dysfunction in vascular endothelial cells

Hydrogen sulfide (H(2)S), the third physiologically relevant gaseous molecule, is recognized increasingly as an anti-inflammatory mediator in various inflammatory conditions. Herein, we explored the effects and mechanisms of sodium hydrosulfide (NaHS, a H(2)S donor) on tumor necrosis factor (TNF)-α-...

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Bibliographic Details
Published in:PloS one 2011-05, Vol.6 (5), p.e19766
Main Authors: Pan, Li-Long, Liu, Xin-Hua, Gong, Qi-Hai, Wu, Dan, Zhu, Yi-Zhun
Format: Article
Language:English
Subjects:
Adhesion
Biology
Cell adhesion
Cell adhesion & migration
Cell Adhesion - drug effects
Cell adhesion molecules
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cytokines
E-selectin
Endothelial cells
Endothelial Cells - drug effects
Endothelial Cells - enzymology
Endothelial Cells - pathology
Endothelium
Enzyme Activation - drug effects
Gene expression
Heme
Heme oxygenase (decyclizing)
Heme Oxygenase-1 - genetics
Heme Oxygenase-1 - metabolism
Humans
Hydrogen
Hydrogen ion concentration
Hydrogen sulfide
I-kappa B Proteins - metabolism
Inflammation
Inflammation - metabolism
Inflammation - pathology
Intercellular adhesion molecule 1
Intercellular Adhesion Molecule-1 - genetics
Intercellular Adhesion Molecule-1 - metabolism
Intracellular Space - drug effects
Intracellular Space - metabolism
Kinases
Medicine
Monocytes
Necrosis
NF-KappaB Inhibitor alpha
NF-κB protein
Nuclear transport
Oxidative stress
Oxygenase
P-selectin
p38 Mitogen-Activated Protein Kinases - metabolism
Pharmacology
Pharmacy
Phosphorylation
Phosphorylation - drug effects
Protein Processing, Post-Translational - drug effects
Protein Transport - drug effects
Proteins
Reactive Oxygen Species - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Rodents
Signal transduction
Signal Transduction - drug effects
Signaling
Sodium
Studies
Sulfide
Sulfides - pharmacology
Transcription Factor RelA - metabolism
Translocation
Tumor Necrosis Factor-alpha - pharmacology
Tumor necrosis factor-TNF
Tumor necrosis factor-α
U937 Cells
Umbilical vein
Up-Regulation - drug effects
Vascular cell adhesion molecule 1
Vascular Cell Adhesion Molecule-1 - genetics
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:Hydrogen sulfide (H(2)S), the third physiologically relevant gaseous molecule, is recognized increasingly as an anti-inflammatory mediator in various inflammatory conditions. Herein, we explored the effects and mechanisms of sodium hydrosulfide (NaHS, a H(2)S donor) on tumor necrosis factor (TNF)-α-induced human umbilical vein endothelial cells (HUVEC) dysfunction. Application of NaHS concentration-dependently suppressed TNF-α-induced mRNA and proteins expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), mRNA expression of P-selectin and E-selectin as well as U937 monocytes adhesion to HUVEC. Western blot analysis revealed that the expression of the cytoprotective enzyme, heme oxygenase-1 (HO-1), was induced and coincident with the anti-inflammatory action of NaHS. Furthermore, TNF-α-induced NF-κB activation assessed by IκBα degradation and p65 phosphorylation and nuclear translocation and ROS production were diminished in cells subjected to treatment with NaHS. H(2)S can exert an anti-inflammatory effect in endothelial cells through a mechanism that involves the up-regulation of HO-1.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0019766