Dendritic cells transfected with scFv from Mab 7.B12 mimicking original antigen gp43 induces protection against experimental Paracoccidioidomycosis

Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naïve T cells, and they also participate in Th-cell educa...

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Bibliographic Details
Published in:PloS one 2011-01, Vol.6 (1), p.e15935-e15935
Main Authors: Ferreira, Karen S, Maranhão, Andrea Q, Garcia, Maria C C, Brígido, Marcelo M, Santos, Suelen S, Lopes, José D, Almeida, Sandro R
Format: Article
Language:English
Subjects:
Analysis
Animal models
Animals
Antibodies
Antibodies, Anti-Idiotypic - therapeutic use
Antibodies, Monoclonal - therapeutic use
Antigens
Antigens, Fungal - immunology
Biology
Bone marrow
Cell growth
Cell proliferation
Cloning
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - transplantation
Fungal Proteins - immunology
Genes
Glycoprotein gp43
Glycoproteins - immunology
Granulocytes
Health aspects
Immunization
Immunoglobulins
Immunotherapy - methods
Lungs
Lymph nodes
Lymphocyte Activation
Lymphocytes
Lymphocytes T
Mice
Mice, Inbred BALB C
Mimicry
Molecular Mimicry
Monoclonal antibodies
Mycoses
Mycosis
Paracoccidioides - immunology
Paracoccidioides brasiliensis
Paracoccidioidomycosis - prevention & control
Paracoccidioidomycosis - therapy
Pathogenesis
Proteins
Single-Chain Antibodies - genetics
Single-Chain Antibodies - therapeutic use
South American blastomycosis
T cells
Tattoos
Therapy
Transfection
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Summary:Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naïve T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0015935